Hirayasu T, Iwamasa T, Kamada Y, Koyanagi Y, Usuda H, Genka K
Department of Pathology, Ryukyu University School of Medicine, Okinawa, Japan.
J Clin Pathol. 1996 Oct;49(10):810-7. doi: 10.1136/jcp.49.10.810.
To compare the incidence of squamous cell carcinoma (SCC) of the lung in Okinawa with that in Niigata on the mainland.
All patients presenting with SCC of the lung in Okinawa and Niigata in 1993 were included in the study. Diagnoses were confirmed by conventional histological examination of paraffin wax sections. Human papillomavirus (HPV) was detected by non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR) amplification with primers specific for the E6 and E7 regions of the HPV genome. PCR products were analysed by Southern and dot blotting.
The incidence of well differentiated SCC of the lung was high in patients from Okinawa compared with moderately and poorly differentiated types, and compared with the incidence of SCC in patients from Niigata. This is despite similar patterns of age, sex (predominatly male), and smoking habit. More patients from Okinawa, however, were positive for HPV DNA by PCR (79%) and NISH (53%). Many patients haboured HPV types 6, 16, and 18. Only 30% of patients from Niigata were positive for HPV DNA by PCR and 20% by NISH. These patients all harboured one HPV type only.
Surprisingly large numbers of patients from Okinawa were positive for HPV DNA. The detection of HPV DNA was strongly associated with well differentiated SCC. This was particularly true for HPV types 6 and 16. There was no correlation between either smoking and detection of HPV DNA, or smoking and histological differentiation.
比较冲绳地区与日本本土新潟地区肺鳞状细胞癌（SCC）的发病率。
纳入1993年在冲绳和新潟诊断为肺SCC的所有患者。诊断通过石蜡切片的常规组织学检查得以确认。采用非同位素原位杂交（NISH）及针对人乳头瘤病毒（HPV）基因组E6和E7区域的引物进行聚合酶链反应（PCR）扩增来检测HPV。PCR产物通过Southern印迹和斑点印迹进行分析。
与中、低分化型相比，冲绳患者中高分化肺SCC的发病率较高，且与新潟患者的SCC发病率相比也是如此。尽管在年龄、性别（以男性为主）和吸烟习惯方面具有相似模式。然而，更多冲绳患者的HPV DNA经PCR检测呈阳性（79%），经NISH检测呈阳性（53%）。许多患者感染了6型、16型和18型HPV。新潟患者中只有30%的HPV DNA经PCR检测呈阳性，20%经NISH检测呈阳性。这些患者均仅感染一种HPV类型。
数量惊人的冲绳患者HPV DNA呈阳性。HPV DNA的检测与高分化SCC密切相关。对于6型和16型HPV尤其如此。HPV DNA检测与吸烟之间以及吸烟与组织学分化之间均无相关性。
