Golbang N, Burnie J P, Klapper P E, Bostock A, Williamson P
Department of Medical Microbiology, Manchester Royal Infirmary.
J Clin Pathol. 1996 Oct;49(10):861-3. doi: 10.1136/jcp.49.10.861.
A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.
描述了一种从血液制品中提取细菌和酵母DNA的新方法，该方法依赖于硫氰酸胍酸提取和蛋白酶K处理。在加标样品中，每0.1 ml血清和血液中，大肠杆菌的灵敏度分别为26和150菌落形成单位（cfu），金黄色葡萄球菌为80和120 cfu，白色念珠菌为20和26 cfu。这与现有方法相比效果良好，适用于有限的临床样本，且不具有病原体特异性。
