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从易于获取的凝血中制备基因组DNA的快速简便方法。

Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood.

作者信息

Kanai N, Fujii T, Saito K, Tokoyama T

机构信息

Department of Pathology, Jichi Medical School, Tochigi, Japan.

出版信息

J Clin Pathol. 1994 Nov;47(11):1043-4. doi: 10.1136/jcp.47.11.1043.

DOI:10.1136/jcp.47.11.1043
PMID:7829682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC503071/
Abstract

A method was developed for the preparation of genomic DNA from clotted blood that is usually discarded after extraction, for other laboratory tests. The method, which involves proteinase K digestion, salt/chloroform extraction and 90% ethanol precipitation of DNA from clotted blood, is rapid, simple, and easy because it does not impose an extra burden on the patient.

摘要

开发了一种从凝血中制备基因组DNA的方法，凝血在提取后通常会被丢弃，用于其他实验室检测。该方法包括蛋白酶K消化、盐/氯仿提取以及从凝血中用90%乙醇沉淀DNA，快速、简单且容易操作，因为它不会给患者带来额外负担。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f91/503071/fcda52ab7f0e/jclinpath00224-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f91/503071/fcda52ab7f0e/jclinpath00224-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f91/503071/fcda52ab7f0e/jclinpath00224-0083-a.jpg

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本文引用的文献

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Nucleic Acids Res. 1991 Mar 11;19(5):1151. doi: 10.1093/nar/19.5.1151.
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