从易于获取的凝血中制备基因组DNA的快速简便方法。
Rapid and simple method for preparation of genomic DNA from easily obtainable clotted blood.
作者信息
Kanai N, Fujii T, Saito K, Tokoyama T
机构信息
Department of Pathology, Jichi Medical School, Tochigi, Japan.
出版信息
J Clin Pathol. 1994 Nov;47(11):1043-4. doi: 10.1136/jcp.47.11.1043.
Abstract
A method was developed for the preparation of genomic DNA from clotted blood that is usually discarded after extraction, for other laboratory tests. The method, which involves proteinase K digestion, salt/chloroform extraction and 90% ethanol precipitation of DNA from clotted blood, is rapid, simple, and easy because it does not impose an extra burden on the patient.
摘要
开发了一种从凝血中制备基因组DNA的方法，凝血在提取后通常会被丢弃，用于其他实验室检测。该方法包括蛋白酶K消化、盐/氯仿提取以及从凝血中用90%乙醇沉淀DNA，快速、简单且容易操作，因为它不会给患者带来额外负担。
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