Redl H, Schlag G, Paul E, Bahrami S, Buurman W A, Strieter R M, Kunkel S L, Davies J, Foulkes R
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria.
Am J Physiol. 1996 Nov;271(5 Pt 2):R1193-8. doi: 10.1152/ajpregu.1996.271.5.R1193.
Tumor necrosis factor (TNF) and interleukin (IL)-1 are two cytokines for which naturally occurring inhibitors have been identified. The present study was undertaken to evaluate the extent to which scavenging of TNF in bacteremia attenuates the plasma levels of IL-1 receptor antagonist (IL-1ra) and soluble TNF receptors (sTNFR). Ten male baboons received 2 x 10(9) colony-forming units/kg live Escherichia coli over 2 h and were subjected to either placebo or anti-TNF antibody (anti-TNF Ab) treatment (1 mg/kg CDP571, Celltech, UK) 2 h before E. coli infusion (observation time: 72h). IL-1ra (range: 50-100 ng/ml) and sTNFR (range: 55kDa, 20-25 ng/ml; 75 kDa, 30-35 ng/ml) release was more sustained than that of IL-1 and TNF and was significantly attenuated by anti-TNF treatment, as were the circulating levels of IL-1, IL-8, and monocyte chemotactic peptide-1 (MCP-1) in the anti-TNF Ab group. We conclude that the increase in circulating natural cytokine modulators observed in nonhuman primate bacteremia is under the partial control of endogenous TNF because it was influenced by anti-TNF pretreatment. This attenuation is comparable to the anti-TNF effect on the chemokine MCP-1.
肿瘤坏死因子（TNF）和白细胞介素（IL）-1是两种已鉴定出天然抑制剂的细胞因子。本研究旨在评估在菌血症中清除TNF能在多大程度上降低IL-1受体拮抗剂（IL-1ra）和可溶性TNF受体（sTNFR）的血浆水平。10只雄性狒狒在2小时内接受2×10⁹菌落形成单位/千克的活大肠杆菌，并在输注大肠杆菌前2小时接受安慰剂或抗TNF抗体（抗TNF Ab）治疗（1毫克/千克CDP571，Celltech，英国）（观察时间：72小时）。IL-1ra（范围：50 - 100纳克/毫升）和sTNFR（范围：55千道尔顿，20 - 25纳克/毫升；75千道尔顿，30 - 35纳克/毫升）的释放比IL-1和TNF更持久，并且抗TNF治疗使其显著减弱，抗TNF Ab组中IL-1、IL-8和单核细胞趋化肽-1（MCP-1）的循环水平也是如此。我们得出结论，在非人类灵长类动物菌血症中观察到的循环天然细胞因子调节剂的增加部分受内源性TNF的控制，因为它受到抗TNF预处理的影响。这种减弱与抗TNF对趋化因子MCP-1的作用相当。
