Tcheng T K, Gillette M U
Neuroscience Program, University of Illinois, Urbana 61801, USA.
J Neurosci Methods. 1996 Nov;69(2):163-9. doi: 10.1016/S0165-0270(96)00039-8.
The fabrication and characteristics of a novel multiunit recording electrode and modified brain slice chamber suitable for long-term recording from brain slices are described. The electrode consisted of an electrolyte-filled glass micropipette with a 20-50 microns thick wax-coated bundle of 5-micron diameter carbon fibers extending 2.5 cm from the tapered end and an AgCl-coated silver wire inserted into the open end and connected to a preamplifier. Both ends of the electrode were sealed with wax to prevent evaporation of the electrolyte. The brain slice was maintained over this extended period in an interface-type brain slice chamber modified to completely surround the slice with medium. Using this electrode, regular 24-h oscillations of spontaneous multiunit activity were recorded for 3 days from a single location in a 500 microns thick rat suprachiasmatic nucleus brain slice. Preliminary data suggest that this novel carbon fiber bundle electrode will be a favorable alternative to traditional metal electrodes for long-term recording of multiunit activity from brain slices.
本文描述了一种新型多单元记录电极和改良脑片腔室的制作及特性，该脑片腔室适用于对脑片进行长期记录。电极由一个充满电解质的玻璃微吸管组成，其带有一束直径为5微米、涂有20至50微米厚蜡的碳纤维，从锥形端延伸2.5厘米，还有一根涂有氯化银的银丝插入开口端并连接到前置放大器。电极两端用蜡密封以防止电解质蒸发。脑片在一个改良的界面型脑片腔室中长时间保持，该腔室经过改进，能使脑片完全被介质包围。使用这种电极，从500微米厚的大鼠视交叉上核脑片的单个位置连续3天记录到了自发多单元活动的规律24小时振荡。初步数据表明，这种新型碳纤维束电极将是传统金属电极在脑片多单元活动长期记录方面的一个有利替代方案。
