Comparison of modified TECRA visual immunoassay with AOAC method 989.14 and reference culture methods 967.25-967.28 for detection of Salmonella in foods and related samples.

A validation study was conducted to compare a new modified version of the TECRA Salmonella Visual immunoassay (Mod. VIA) with the original immunoassay (AOAC Method 989.14) and the reference culture methods (967.25-967.28). The Mod. VIA was supplemented with polyclonal antibodies to Salmonella pullorum and S. gallinarum, and compared favorably with AOAC Method 989.14 in sensitivity, inclusivity, and exclusivity studies. The ability of the Mod. VIA to recover Salmonella from artificially contaminated samples was not significantly different from that of the reference method. It is recommended that the Mod. VIA be adopted as Official First Action.