Somani S M, Husain K, Diaz-Phillips L, Lanzotti D J, Kareti K R, Trammell G L
Department of Pharmacology, Southern Illinois University, School of Medicine, Springfield 62794-1222, USA.
Alcohol. 1996 Nov-Dec;13(6):603-10. doi: 10.1016/s0741-8329(96)00075-4.
This study investigates the effect of ethanol ingestion on antioxidant enzymes (AOE) and lipid peroxidation (malondialdehyde, (MDA) in different brain regions of the rat after acute exercise. Acute exercise (100% VO2max) significantly increased glutathione peroxidase (GSH-Px) activity and decreased glutathione reductase (GR) activity in the cerebral cortex. Acute exercise significantly increased MDA level in the corpus striatum. Ethanol (20%) (1.6 g/kg, PO) significantly increased MDA level in the cerebral cortex. Ethanol also significantly increased superoxide dismutase (SOD) activity in the cortex and catalase (CAT), GSH-Px, and GR activities in the corpus striatum. Ethanol significantly augmented CAT activity in the medulla and GSH-Px activity in the hypothalamus. However, CAT activity significantly decreased in the hypothalamus after ethanol ingestion. The combination significantly increased GSH-Px activity in the hypothalamus, SOD activity in the cortex, GR activity in the striatum, and MDA level in the medulla. In conclusion, the cerebral cortex, striatum medulla, and hypothalamus reacted differentially in response to ethanol as well as to acute exercise-induced oxidative stress whereas the combination moderated the changes in AOE activity in specific brain regions.
本研究调查了急性运动后乙醇摄入对大鼠不同脑区抗氧化酶（AOE）和脂质过氧化（丙二醛，MDA）的影响。急性运动（100%最大摄氧量）显著增加了大脑皮层中谷胱甘肽过氧化物酶（GSH-Px）的活性，并降低了谷胱甘肽还原酶（GR）的活性。急性运动显著增加了纹状体中的MDA水平。乙醇（20%）（1.6 g/kg，口服）显著增加了大脑皮层中的MDA水平。乙醇还显著增加了皮层中超氧化物歧化酶（SOD）的活性以及纹状体中过氧化氢酶（CAT）、GSH-Px和GR的活性。乙醇显著增强了延髓中的CAT活性以及下丘脑中的GSH-Px活性。然而，摄入乙醇后下丘脑中的CAT活性显著降低。乙醇与急性运动联合显著增加了下丘脑中的GSH-Px活性、皮层中的SOD活性、纹状体中的GR活性以及延髓中的MDA水平。总之，大脑皮层、纹状体、延髓和下丘脑对乙醇以及急性运动诱导的氧化应激反应不同，而二者联合可调节特定脑区中AOE活性的变化。
