Martínez P, Lejarazu R O, Eiros J M, Benito J D, Rodríguez-Torres A
Microbiology Laboratory Service, University Hospital, Valladolid, Spain.
Eur J Clin Microbiol Infect Dis. 1996 Oct;15(10):810-3. doi: 10.1007/BF01701524.
The detection of specific antibodies against human immunodeficiency virus (HIV) was tested by dot blot enzyme immunoassay in 95 urine samples from 72 individuals infected with HIV and 23 seronegative individuals. Western blot of paired serum samples from these same individuals was used as the gold standard. The dot blot tested had a sensitivity of 97.2% and a specificity of 100%; only two samples from HIV-infected individuals at Centers for Disease Control (CDC) stages II and IV were non-reactive. Reactive and discrepant samples (serum/urine) were confirmed by Western blot, which had a sensitivity of 98.6% and a specificity of 100%. The most commonly observed Western blot reactivity pattern in urine samples included bands against three groups of HIV structural proteins (ENV, POL, and GAG). The results indicate that urine can be used in screening for HIV antibodies in epidemiological studies of high-prevalence populations, though it is not recommended for individualized diagnostic purposes.
采用斑点印迹酶免疫分析法对72例感染人类免疫缺陷病毒（HIV）的个体和23例血清学阴性个体的95份尿液样本进行了HIV特异性抗体检测。将这些个体的配对血清样本进行蛋白质印迹分析作为金标准。所检测的斑点印迹法灵敏度为97.2%，特异性为100%；疾病控制中心（CDC）II期和IV期HIV感染个体中只有两份样本无反应。通过蛋白质印迹法对反应性和不一致的样本（血清/尿液）进行确认，其灵敏度为98.6%，特异性为100%。尿液样本中最常见的蛋白质印迹反应模式包括针对三组HIV结构蛋白（ENV、POL和GAG）的条带。结果表明，尿液可用于高流行人群的HIV抗体流行病学筛查研究，但不推荐用于个体诊断。
