Matsiota P, Druet P, Dosquet P, Guilbert B, Avrameas S
Département d'Immunologie, Institut Pasteur, Paris, France.
Clin Exp Immunol. 1987 Jul;69(1):79-88.
We have tested the sera of 25 patients with systemic lupus erythematosus (SLE) for antibody activity against a panel of six antigens: DNA, TNP, actin, tubulin, myosin, albumin. Eluates from renal biopsy tissue were also tested. Sera from patients with lupus nephritis were found to contain high titres of IgA antibodies directed against the antigens of the panel, and marked IgG anti-DNA and anti-TNP antibody activity. The IgG anti-TNP antibodies isolated from SLE serum by affinity chromatography on a TNP-immunoadsorbent, were also found to possess anti-DNA activity. Kidney eluates obtained from biopsy specimens of SLE patients contained IgG antibodies strictly specific for DNA in three out of the nine patients tested, while three eluates from the remaining six patients reacted with DNA and TNP and three with DNA and all the other antigens of the panel. These results strongly suggest that in SLE sera there are at least three populations of circulating anti-DNA antibodies: those strictly specific for DNA, those recognizing DNA and TNP and those recognizing DNA and other macromolecules. Furthermore, because six out of nine of the eluates contained antibodies with an absolute or restricted specificity for DNA, this suggests that these antibodies are more often pathogenic than the polyspecific ones recognizing DNA and other macromolecules.
我们检测了25例系统性红斑狼疮（SLE）患者的血清针对一组六种抗原（DNA、三硝基苯（TNP）、肌动蛋白、微管蛋白、肌球蛋白、白蛋白）的抗体活性。还检测了肾活检组织的洗脱液。发现狼疮性肾炎患者的血清含有高滴度的针对该组抗原的IgA抗体，以及显著的IgG抗DNA和抗TNP抗体活性。通过在TNP免疫吸附剂上进行亲和层析从SLE血清中分离出的IgG抗TNP抗体，也被发现具有抗DNA活性。从9例接受检测的SLE患者的活检标本中获得的肾洗脱液，有3例含有对DNA具有严格特异性的IgG抗体，而其余6例患者的3份洗脱液与DNA和TNP发生反应，3份与DNA及该组的所有其他抗原发生反应。这些结果有力地表明，在SLE血清中至少存在三种循环抗DNA抗体群体：那些对DNA具有严格特异性的抗体、那些识别DNA和TNP的抗体以及那些识别DNA和其他大分子的抗体。此外，由于9份洗脱液中有6份含有对DNA具有绝对或受限特异性的抗体，这表明这些抗体比识别DNA和其他大分子的多特异性抗体更常具有致病性。
