Gunn-Moore D A, Pearson G R, Harbour D A, Whiting C V
Department of Veterinary Pathology, Faculty of Agriculture, University of Tokyo, Japan.
Vet Pathol. 1996 Nov;33(6):699-703. doi: 10.1177/030098589603300610.
This report describes the clinical, pathological, immunocytochemical, and in situ hybridization characteristics of encephalitis associated with feline immunodeficiency virus (FIV) infection in a 4-year-old domestic cat. Lesions were identified throughout the brain, affecting the cerebrum, medulla, and cervical spinal cord. Perivascular lymphocytic cuffing, gliosis, and white matter vacuolation were most severe in the cerebrum, affecting the white matter and the deep laminae of the grey matter. Gemistocytes were prominent, and many bizarre cells with large, sometimes multinucleate, hyperchromatic nuclei were evident. Immunostaining with antibody specific for FIV p24 nucleocapsid protein produced staining in the gemistocytes and glial cells of the white matter. In situ hybridization with a 327-base pair fragment of the FIV gag gene produced staining that was most intense in the white matter and gemistocytes of the deep laminae of the grey matter. These findings indicated localization of FIV infection within the cerebrum, and the detection of FIV RNA by in situ hybridization confirms the infection as active.
本报告描述了一只4岁家猫感染猫免疫缺陷病毒（FIV）后发生脑炎的临床、病理、免疫细胞化学及原位杂交特征。脑部各处均发现病变，累及大脑、延髓和颈脊髓。血管周围淋巴细胞套袖状浸润、胶质细胞增生和白质空泡化在大脑最为严重，影响白质和灰质深层。肥胖型星形胶质细胞突出，可见许多具有大的、有时多核的、深染细胞核的奇异细胞。用针对FIV p24核衣壳蛋白的特异性抗体进行免疫染色，在白质的肥胖型星形胶质细胞和神经胶质细胞中产生染色。用FIV gag基因的327个碱基对片段进行原位杂交，在灰质深层的白质和肥胖型星形胶质细胞中产生的染色最为强烈。这些发现表明FIV感染定位于大脑，原位杂交检测到FIV RNA证实感染处于活跃状态。
