番茄细菌性斑点病中基因对基因特异性的分子基础

Molecular Basis of Gene-for-Gene Specificity in Bacterial Speck Disease of Tomato.

作者信息

Scofield SR, Tobias CM, Rathjen JP, Chang JH, Lavelle DT, Michelmore RW, Staskawicz BJ

机构信息

S. R. Scofield, J. P. Rathjen, J. H. Chang, D. T. Lavelle, NSF Center for Engineering Plants for Resistance Against Pathogens (CEPRAP), University of California, Davis, CA 95616. C. M. Tobias, Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720. R. W. Michelmore, NSF Center for Engineering Plants for Resistance Against Pathogens and Department of Vegetable Crops, University of California, Davis, CA 95616. B. J. Staskawicz, NSF Center for Engineering Plants for Resistance Against Pathogens and Department of Plant and Microbial Biology, University of California, Davis, CA 94720.

出版信息

Science. 1996 Dec 20;274(5295):2063-5. doi: 10.1126/science.274.5295.2063.

DOI:10.1126/science.274.5295.2063

PMID:8953034

Abstract

Transient expression of the Pseudomonas syringae avirulence gene avrPto in plant cells resulted in a Pto-dependent necrosis. The AvrPto avirulence protein was observed to interact directly with the Pto resistance protein in the yeast two-hybrid system. Mutations in the Pto and avrPto genes which reduce in vivo activity had parallel effects on association in the two-hybrid assay. These data suggest that during infection the pathogen delivers AvrPto into the plant host cell and that resistance is specified by direct interaction of Pto with AvrPto.

摘要

丁香假单胞菌无毒基因avrPto在植物细胞中的瞬时表达导致了依赖Pto的细胞坏死。在酵母双杂交系统中，观察到AvrPto无毒蛋白与Pto抗性蛋白直接相互作用。Pto和avrPto基因中降低体内活性的突变对双杂交试验中的相互作用有相似的影响。这些数据表明，在感染过程中，病原菌将AvrPto传递到植物宿主细胞中，并且抗性是由Pto与AvrPto的直接相互作用决定的。