双孢蘑菇表达序列标签的分离及其染色体定位
Isolation of expressed sequence tags of Agaricus bisporus and their assignment to chromosomes.
作者信息
Sonnenberg A S, de Groot P W, Schaap P J, Baars J J, Visser J, Van Griensven L J
机构信息
Mushroom Experimental Station, Horst, The Netherlands.
出版信息
Appl Environ Microbiol. 1996 Dec;62(12):4542-7. doi: 10.1128/aem.62.12.4542-4547.1996.
Abstract
The genome of the cultivated basidiomycete Agaricus bisporus Horst U1 and of its homokaryotic parents has been characterized by using an optimized method of pulsed-field gel electrophoresis. Expressed sequence tags obtained as expressed cDNAs from a primordial tissue-derived cDNA library and a number of previously isolated genes were used to identify the individual chromosomes of the parental lines of Horst U1. The genome consists of 13 chromosomes, and its total size is 31 Mb. For those chromosomes that could not be resolved by contour-clamped homogeneous electric field electrophoresis, the segregation of marker genes was studied in a set of 86 homokaryotic offspring of Horst U1. At least two markers were assigned to each individual chromosome. In this way all individual chromosomes were unequivocally identified. The large size difference observed between the homologous chromosomes IX, harboring the rDNA repeat, was shown to be largely due to a higher copy number of rDNA in parental strain H97 than in parental strain H39.
摘要
利用优化的脉冲场凝胶电泳方法，对栽培担子菌双孢蘑菇霍斯特U1及其同核体亲本的基因组进行了表征。从原始组织来源的cDNA文库中获得的表达序列标签以及一些先前分离的基因，被用于鉴定霍斯特U1亲本系的各个染色体。该基因组由13条染色体组成，总大小为31兆碱基对。对于那些无法通过轮廓夹钳均匀电场电泳分辨的染色体，在霍斯特U1的一组86个同核体后代中研究了标记基因的分离情况。每个单独的染色体至少分配了两个标记。通过这种方式，所有单独的染色体都得到了明确鉴定。观察到携带rDNA重复序列的同源染色体IX之间存在较大的大小差异，这在很大程度上是由于亲本菌株H97中的rDNA拷贝数高于亲本菌株H39。
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