利用携带嗜热栖热菌木糖A基因（该基因表达一种活性木糖（葡萄糖）异构酶）的酿酒酵母对木糖进行乙醇发酵。
Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase.
作者信息
Walfridsson M, Bao X, Anderlund M, Lilius G, Bülow L, Hahn-Hägerdal B
机构信息
Department of Applied Microbiology, Lund Institute of Technology/Lund University, Sweden.
出版信息
Appl Environ Microbiol. 1996 Dec;62(12):4648-51. doi: 10.1128/aem.62.12.4648-4651.1996.
Abstract
The Thermus thermophilus xylA gene encoding xylose (glucose) isomerase was cloned and expressed in Saccharomyces cerevisiae under the control of the yeast PGK1 promoter. The recombinant xylose isomerase showed the highest activity at 85 degrees C with a specific activity of 1.0 U mg-1. A new functional metabolic pathway in S. cerevisiae with ethanol formation during oxygen-limited xylose fermentation was demonstrated. Xylitol and acetic acid were also formed during the fermentation.
摘要
编码木糖（葡萄糖）异构酶的嗜热栖热菌木糖A基因在酵母PGK1启动子的控制下克隆并在酿酒酵母中表达。重组木糖异构酶在85℃时表现出最高活性，比活性为1.0 U mg-1。证明了酿酒酵母中一种新的功能代谢途径，即在限氧木糖发酵过程中有乙醇生成。发酵过程中还形成了木糖醇和乙酸。
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Ethanolic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose (glucose) isomerase.利用携带嗜热栖热菌木糖A基因（该基因表达一种活性木糖（葡萄糖）异构酶）的酿酒酵母对木糖进行乙醇发酵。
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