Torihashi S, Yoshida H, Nishikawa S, Kunisada T, Sanders K M
Department of Physiology and Cell Biology, University of Nevada, School of Medicine, Reno 89557, USA.
Brain Res. 1996 Nov 4;738(2):323-8. doi: 10.1016/s0006-8993(96)00935-3.
The cells that express Steel factor (SLF) in the gastrointestinal (GI) tract were studied using SLF-lacZ transgenic mice. Expression, detected by beta-galactosidase histochemistry, was evident in cells between the circular and longitudinal muscle layers in the GI tract. Double staining with antibodies specific for the neural markers, PGP 9.5, MAP2 and c-Ret, showed that SLF-lacZ positive cells were enteric neurons. Enteroglia did not express SLF-lacZ. The distribution of expressing cells was complimentary to the expression of c-Kit in myenteric interstitial cells.
利用Steel因子（SLF）-lacZ转基因小鼠对胃肠道（GI）中表达Steel因子的细胞进行了研究。通过β-半乳糖苷酶组织化学检测到的表达在胃肠道环形肌层和纵行肌层之间的细胞中很明显。用针对神经标志物PGP 9.5、MAP2和c-Ret的特异性抗体进行双重染色，结果显示SLF-lacZ阳性细胞为肠神经元。肠胶质细胞不表达SLF-lacZ。表达细胞的分布与肌间神经丛间质细胞中c-Kit的表达互补。
