Barth P J, Weingärtner K, Köhler H H, Bittinger A
Department of Pathology, Philipps-Universität, Marburg/Lahn, Germany.
Hum Pathol. 1996 Dec;27(12):1306-10. doi: 10.1016/s0046-8177(96)90342-1.
Prostatic carcinoma obtained from 41 patients (pT2N0, 5; pT2N+, 2; pT3N0, 16; pT3N+, 16; pT4N0, 1; and pT4N+, 1) ranging from 45 to 79 years of age were investigated in the present study. A total of 410 tumor areas of different grades were analyzed (G1, n = 116; G2, n = 98; and G3, n = 196). Vascular structures were labeled immunohistochemically using factor-VIII-associated antigen. The vascular surface density (VSD), the microvessel number (NVES), and the maximum microvessel number (NVES-MAX) were assessed by means of stereology, and the results were related to tumor stage, nodal status, and grade of differentiation. NVES and NVES-MAX showed a significant increase with rising pT stage ranging from 25.5 +/- 1.48 in controls to 135.0 +/- 5.5 microvessels/mm2 in pT4 tumors. Discrimination of different pT stages was more accurate with NVES-MAX. The VSD was significantly higher in pT2 tumors compared with controls, whereas there were no significant differences between pT3 tumors, pT4 tumors, and controls, although the values in pT3 and pT4 tumors were distinctly lower than in pT2 tumors (P < .05). The VSD and the NVES were not able to discriminate between the pN0 and the pN+ group. Both parameters were slightly higher in patients with metastatic disease. Only NVES-MAX values differed between the two groups with an average of additional 21 microvessels/mm2 in the pN+ group (P < .05). Concerning the grade of tumor differentiation the VSD continuously decreased from G1 (14.58 +/- 2.24 mm(-1) to G3 tumor areas (5.41 +/- 1.46 mm(-1). Only G1 tumors showed significant differences compared with controls (6.65 +/- 0.38 mm(-1). The NVES increased with rising tumor grade with significant differences between all four groups ranging from 25.5 +/- 1.5 in controls to 136.9 +/- 37.2 microvessels/mm2 in pT4 tumors.
本研究调查了41例前列腺癌患者（pT2N0，5例；pT2N+，2例；pT3N0，16例；pT3N+，16例；pT4N0，1例；pT4N+，1例），年龄在45至79岁之间。共分析了410个不同分级的肿瘤区域（G1，n = 116；G2，n = 98；G3，n = 196）。使用因子VIII相关抗原通过免疫组织化学标记血管结构。通过体视学评估血管表面密度（VSD）、微血管数量（NVES）和最大微血管数量（NVES-MAX），并将结果与肿瘤分期、淋巴结状态和分化程度相关联。NVES和NVES-MAX显示随着pT分期升高而显著增加，从对照组的25.5±1.48增加到pT4肿瘤的135.0±5.5个微血管/mm2。NVES-MAX对不同pT分期的区分更准确。与对照组相比，pT2肿瘤的VSD显著更高，而pT3肿瘤、pT4肿瘤与对照组之间无显著差异，尽管pT3和pT4肿瘤的值明显低于pT2肿瘤（P <.05）。VSD和NVES无法区分pN0和pN+组。这两个参数在有转移疾病的患者中略高。只有NVES-MAX值在两组之间有所不同，pN+组平均额外增加21个微血管/mm2（P <.05）。关于肿瘤分化程度，VSD从G1（14.58±2.24 mm-1）持续下降到G3肿瘤区域（5.41±1.46 mm-1）。只有G1肿瘤与对照组相比有显著差异（6.65±0.38 mm-1）。NVES随着肿瘤分级升高而增加，所有四组之间有显著差异，从对照组的25.5±1.5增加到pT4肿瘤的136.9±37.2个微血管/mm2。
