Polson A M, Southard G L, Dunn R L, Yewey G L, Godowski K C, Polson A P, Fulfs J C, Laster L
Atrix Laboratories, Fort Collins, Co., USA.
J Periodontol. 1996 Nov;67(11):1176-84. doi: 10.1902/jop.1996.67.11.1176.
The present study evaluated the clinical response of periodontal pockets in beagle dogs after treatment with a biodegradable delivery system containing 10% doxycycline hyclate (ABDS-D). Eight adult, female beagle dogs had generalized, severe periodontitis with plaque and calculus-laden pockets. In each animal, 3 teeth with multiple pocket sites > or = 4 mm (mean depth = 6.0 mm) associated with attachment loss (mean = 5.4 mm) and which bled on probing (mean score = 2.5) were treated with a single application of either ABDS-D (experimental group) or the delivery system alone without the doxycycline (control group). Residual polymer was removed at day 7. Bioassay of doxycycline in gingival crevicular fluid associated with presence of ABDS-D gave mean levels of bioactivity of approximately 250 micrograms/ml. Levels of bioactive doxycycline were detected for approximately 7 days after ABDS-D removal. Periodontal maintenance consisted of thrice-weekly toothbrushing the treated sites. Clinical responses were evaluated at 2 weeks, and at bi-weekly intervals thereafter for 4 months. Analyses of the data from the control group showed that there was only slight clinical improvement. In contrast, in the experimental group, bleeding on probing and probing depths were significantly reduced from baseline at all post-treatment time points. At 1 month, mean probing depth reduction was 2.4 mm and this was maintained at 4 months (mean reduction = 2.5 mm). These probing depth reductions occurred primarily through gain of clinical attachment which was 2.0 mm at 4 months. Bleeding had been virtually eliminated (mean = 0.2). It was concluded that, for the beagle dogs with severely infected periodontal pockets in this study, treatment with subgingival doxycycline using the delivery system resulted in substantial improvement in periodontal health.
本研究评估了含10%盐酸多西环素的可生物降解给药系统（ABDS-D）治疗后，比格犬牙周袋的临床反应。八只成年雌性比格犬患有广泛性重度牙周炎，牙周袋内有菌斑和牙石。在每只动物中，选取3颗有多个深度≥4 mm（平均深度 = 6.0 mm）的牙周袋部位、伴有附着丧失（平均 = 5.4 mm）且探诊出血（平均评分 = 2.5）的牙齿，单次应用ABDS-D（实验组）或不含多西环素的单纯给药系统（对照组）进行治疗。在第7天去除残留聚合物。与ABDS-D存在相关的龈沟液中多西环素的生物测定显示，生物活性平均水平约为250微克/毫升。去除ABDS-D后约7天可检测到生物活性多西环素水平。牙周维护包括每周三次对治疗部位进行刷牙。在2周时评估临床反应，此后每两周评估一次，持续4个月。对照组数据分析表明，仅有轻微的临床改善。相比之下，在实验组中，所有治疗后时间点的探诊出血和探诊深度均较基线显著降低。在1个月时，平均探诊深度减少2.4 mm，在4个月时维持该水平（平均减少 = 2.5 mm）。这些探诊深度的减少主要是通过临床附着增加实现的，4个月时为2.0 mm。出血几乎消除（平均 = 0.2）。得出的结论是，对于本研究中患有严重感染牙周袋的比格犬，使用该给药系统进行龈下多西环素治疗可使牙周健康状况得到显著改善。
