Microbial contamination of pharmacies and hospital wards environment and its influence on the purity of prescription preparations during their production process as well as their application.

Microbiological contamination of such environmental factors as air, surfaces of equipment, walls, floors and personnel gloves in two hospital pharmacies and different paediatric hospital wards were tested. Most surfaces in the areas, where the sterile preparations (eye dosage forms) are prepared, turned out to meet the hygienic requirements, although some bacteria belonging to Staphylococci, Pseudomonas, Acinetobacter were detected. The considerable number of microorganisms from 10(2) up to 10(6) cells on 25 cm2 was found on personnel hands and on surfaces of the rooms, where the nonsterile prescription preparations for external and oral use are prepared. Among the identified microorganisms, S. aureus, Enterobacter, Proteus and Moraxella were also observed. However, most opportunistic pathogens like E. coli, Enterobacter, Klebsiella, Citrobacter, Proteus and Ps. aeruginosa were found in the wards in lower numbers (10(0)-10(2). The negative influence of the environment contamination on the purity of prescription preparations, especially during their application in the hospital wards, was observed.