Strettoi E, Masland R H
Istituto di Neurofisiologia del Consiglio Nazionale delle Ricerche, Pisa, Italy.
Proc Natl Acad Sci U S A. 1996 Dec 10;93(25):14906-11. doi: 10.1073/pnas.93.25.14906.
The three largest known populations of amacrine cells in the rabbit retina were stained with fluorescent probes in whole mounts and counted at a series of retinal eccentricities. The retinas were counterstained using a fluorescent DNA-binding molecule and the total number of nuclei in the inner nuclear layer were counted in confocal sections. From the total number of inner nuclear layer cells and the known fraction of them occupied by amacrine cells, the fraction of amacrine cells made up by the stained populations could be calculated. Starburst cells made up 3%, indoleamine-accumulating cells made up 4%, and AII cells made up 11% of all amacrine cells. By referring four smaller populations of amacrine cells to the number of indoleamine-accumulating cells, they were estimated to make up 4% of all amacrine cells. Thus, 78% of all amacrine cells in the rabbit's retina are known only from isolated examples, if at all. This proportion is similar in the retinas of the mouse, cat, and monkey. It is likely that a substantial fraction of the local circuit neurons present in other regions of the central nervous system are also invisible as populations to current techniques.
用荧光探针将兔视网膜中已知的三大类无长突细胞群体在整装片中染色，并在一系列视网膜离心度下进行计数。视网膜用荧光DNA结合分子复染，在内核层共聚焦切片中对细胞核总数进行计数。根据内核层细胞总数以及无长突细胞所占的已知比例，可计算出染色群体构成的无长突细胞比例。星爆细胞占所有无长突细胞的3%，吲哚胺积累细胞占4%，AII细胞占11%。通过将另外四类较小的无长突细胞群体与吲哚胺积累细胞数量进行对比，估计它们占所有无长突细胞的4%。因此，兔子视网膜中78%的无长突细胞仅从个别例子中得知，即便有所了解也极为有限。在小鼠、猫和猴子的视网膜中这一比例相似。很可能中枢神经系统其他区域存在的相当一部分局部回路神经元群体，对于当前技术而言同样不可见。
