Zasukhina G D, Sinel'shchikova T A, Vasil'eva I M, Meliksetova I A, Alekhina N I, Semiachkina A N
Genetika. 1996 Nov;32(11):1592-5.
Two lines of fibroblasts isolated by skin biopsy from patients with homocystinuria were characterized as repair-defective cells. Preirradiation of these cells at low doses (0.1 Gy) and irradiation at high doses demonstrated their decreased ability to form radioadaptive response, in comparison with normal cells. Radioadaptive response did not differ from that of normal cells after chemical treatment with 4NQO and NG used as damaging agents. The method for detection of DNA breaks by means of hydroxyapatite column chromatography of cell lysates and the method of determining sister chromatid exchanges (SCE) were used.
从同型胱氨酸尿症患者皮肤活检分离出的两株成纤维细胞被鉴定为修复缺陷细胞。与正常细胞相比，这些细胞在低剂量（0.1 Gy）预照射后再进行高剂量照射，显示出其形成辐射适应性反应的能力下降。在用4NQO和NG作为损伤剂进行化学处理后，辐射适应性反应与正常细胞无异。使用了通过细胞裂解物的羟基磷灰石柱色谱法检测DNA断裂的方法以及测定姐妹染色单体交换（SCE）的方法。
