使用双插入化合物对DNA插入发色团之间的共振能量转移问题进行重新审视。
A reexamination of the problem of resonance energy transfer between DNA intercalated chromophores using bisintercalating compounds.
作者信息
Le Bret M, Le Pecq J B, Barbet J, Roques B P
出版信息
Nucleic Acids Res. 1977;4(5):1361-79. doi: 10.1093/nar/4.5.1361.
Abstract
The rate of energy transfer between DNA intercalated ethidium cations calculated by Paoletti and Le Pecq1 using the Forster theory differs from the measured one by a factor of twenty two, if the proper geometrical factors are taken into account. By changing some of the parameters used in the calculation, the discrepancy can be reduced but not eliminated. This led us to the study of other systems where experimental and calculated results can be more directly compared. The apparent rate of energy transfer between ethidium and one of its non fluorescent analogues and between various pairs of intercalated chromophores has been studied. The fluorescence anisotropy decay of acridine dimers in glycerol or bisintercalated in DNA has been measured. These studies show that the Forster theory of energy transfer does not apply to the case of identical chromophores when they are relatively close to each other.
摘要
如果考虑适当的几何因素，帕奥莱蒂和勒佩克1运用福斯特理论计算得出的嵌入DNA的乙锭阳离子之间的能量转移速率，与测量值相差22倍。通过改变计算中使用的一些参数，这种差异可以减小，但无法消除。这促使我们研究其他能够更直接比较实验结果和计算结果的体系。我们研究了乙锭与其一种非荧光类似物之间以及各种嵌入发色团对之间的表观能量转移速率。测量了吖啶二聚体在甘油中或双嵌入DNA中的荧光各向异性衰减。这些研究表明，当相同发色团彼此相对靠近时，福斯特能量转移理论并不适用。
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