使用活细胞染色通过流式细胞术对真菌进行快速药敏试验。
Rapid susceptibility testing of fungi by flow cytometry using vital staining.
作者信息
Wenisch C, Linnau K F, Parschalk B, Zedtwitz-Liebenstein K, Georgopoulos A
机构信息
Department of Infectious Disease, Internal Medicine I, University Hospital of Vienna, Austria.
出版信息
J Clin Microbiol. 1997 Jan;35(1):5-10. doi: 10.1128/jcm.35.1.5-10.1997.
Abstract
A 1-h assay for antifungal susceptibility testing measuring the impairment of fungal metabolic activity was developed. Yeast viability was analyzed by flow cytometry with a novel fluorescent probe, FUN-1, which emits a red fluorescence when the yeast is metabolically active. For nine Candida albicans strains tested, this method yielded results comparable to those obtained by the standard M27 procedure for amphotericin B, flucytosine, fluconazole, and ketoconazole. Whether the flow cytometry antifungal susceptibility test results correlate with the in vivo activities of the drugs remains to determined.
摘要
开发了一种用于抗真菌药敏试验的1小时检测方法，该方法可测量真菌代谢活性的损害。使用一种新型荧光探针FUN-1通过流式细胞术分析酵母活力，当酵母具有代谢活性时，该探针会发出红色荧光。对于所测试的9株白色念珠菌菌株，该方法产生的结果与通过标准M27程序获得的两性霉素B、氟胞嘧啶、氟康唑和酮康唑的结果相当。流式细胞术抗真菌药敏试验结果是否与药物的体内活性相关仍有待确定。
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Quality control guidelines for National Committee for Clinical Laboratory Standards recommended broth macrodilution testing of amphotericin B, fluconazole, and flucytosine.美国国家临床实验室标准委员会的质量控制指南推荐采用肉汤稀释法对两性霉素B、氟康唑和氟胞嘧啶进行检测。
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