Rhoden D L, Hancock G A, Miller J M
Nosocomial Pathogens Laboratory Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
J Clin Microbiol. 1993 Mar;31(3):490-3. doi: 10.1128/jcm.31.3.490-493.1993.
A numerical-code system for the reference identification of Staphylococcus species, Stomatococcus mucilaginosus, and Micrococcus species was established by using a selected panel of conventional biochemicals. Results from 824 cultures (289 eye isolate cultures, 147 reference strains, and 388 known control strains) were used to generate a list of 354 identification code numbers. Each six-digit code number was based on results from 18 conventional biochemical reactions. Seven milliliters of purple agar base with 1% sterile carbohydrate solution added was poured into 60-mm-diameter agar plates. All biochemical tests were inoculated with 1 drop of a heavy broth suspension, incubated at 35 degrees C, and read daily for 3 days. All reactions were read and interpreted by the method of Kloos et al. (G. A. Hebert, C. G. Crowder, G. A. Hancock, W. R. Jarvis, and C. Thornsberry, J. Clin. Microbiol. 26:1939-1949, 1988; W. E. Kloos and D. W. Lambe, Jr., P. 222-237, in A. Balows, W. J. Hansler, Jr., K. L. Herrmann, H. D. Isenberg, and H. J. Shadomy, ed., Manual of Clinical Microbiology, 5th ed., 1991). This modified reference identification method was 96 to 98% accurate and could have value in reference and public health laboratory settings.
通过使用一组选定的传统生化试剂，建立了一种用于葡萄球菌属、黏性口腔球菌和微球菌属参考鉴定的数字编码系统。利用824份培养物（289份眼部分离培养物、147份参考菌株和388份已知对照菌株）的结果生成了一份包含354个识别编码的列表。每个六位编码基于18种传统生化反应的结果。将7毫升添加了1%无菌碳水化合物溶液的紫色琼脂基础培养基倒入直径60毫米的琼脂平板中。所有生化试验均接种1滴浓肉汤悬液，在35℃下培养，并连续3天每日观察结果。所有反应均按照克洛斯等人的方法进行判读和解释（G. A. 赫伯特、C. G. 克劳德、G. A. 汉考克、W. R. 贾维斯和C. 索恩斯伯里，《临床微生物学杂志》26:1939 - 1949，1988；W. E. 克洛斯和D. W. 兰贝， Jr.，第222 - 237页，载于A. 巴洛斯、W. J. 汉斯勒， Jr.、K. L. 赫尔曼、H. D. 伊森伯格和H. J. 沙多米编，《临床微生物学手册》，第5版，1991）。这种改良的参考鉴定方法准确率为96%至98%，在参考和公共卫生实验室环境中可能具有价值。
