• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

比较乙二胺四乙酸（EDTA）管和枸橼酸-柠檬酸-葡萄糖（acid-citrate-dextrose）采集管在储存前后检测白细胞中巨细胞病毒抗原血症和感染性的效果。

Comparison of EDTA and acid-citrate-dextrose collection tubes for detection of cytomegalovirus antigenemia and infectivity in leukocytes before and after storage.

作者信息

Landry M L, Cohen S, Huber K

机构信息

Clinical Virology Laboratory, Yale New Haven Hospital, Connecticut 06504, USA.

出版信息

J Clin Microbiol. 1997 Jan;35(1):305-6. doi: 10.1128/jcm.35.1.305-306.1997.

DOI:10.1128/jcm.35.1.305-306.1997
PMID:8968934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229565/
Abstract

Duplicate blood samples collected in EDTA and acid-citrate-dextrose (ACD) were compared by cytomegalovirus (CMV) pp65 antigenemia and CMV infectivity on the day of sample collection and after 1 and 2 days of storage at 4 degrees C. No significant difference was detected between EDTA and ACD. However, CMV antigenemia was more sensitive than culture at all time points tested.

摘要

通过巨细胞病毒（CMV）pp65抗原血症和CMV感染性，对采集于乙二胺四乙酸（EDTA）和枸橼酸-柠檬酸-葡萄糖（ACD）中的重复血样在采集当天以及在4℃储存1天和2天后进行比较。未检测到EDTA和ACD之间存在显著差异。然而，在所有测试时间点，CMV抗原血症比培养更敏感。

相似文献

1
Comparison of EDTA and acid-citrate-dextrose collection tubes for detection of cytomegalovirus antigenemia and infectivity in leukocytes before and after storage.比较乙二胺四乙酸（EDTA）管和枸橼酸-柠檬酸-葡萄糖（acid-citrate-dextrose）采集管在储存前后检测白细胞中巨细胞病毒抗原血症和感染性的效果。
J Clin Microbiol. 1997 Jan;35(1):305-6. doi: 10.1128/jcm.35.1.305-306.1997.
2
Comparison of ethylenediaminetetraacetic acid and sodium citrate as anticoagulants in collection of samples for cytomegalovirus pp65 antigen detection in renal transplant recipients with suspected cytomegalovirus disease.在疑似巨细胞病毒病的肾移植受者中，比较乙二胺四乙酸和柠檬酸钠作为抗凝剂在采集用于巨细胞病毒pp65抗原检测的样本时的效果。
J Virol Methods. 2008 Feb;147(2):319-21. doi: 10.1016/j.jviromet.2007.09.019. Epub 2007 Nov 9.
3
Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.通过空斑试验、pp65抗原血症试验和聚合酶链反应比较肝素和乙二胺四乙酸（EDTA）转运管在白细胞中检测巨细胞病毒的效果
J Clin Microbiol. 1994 Oct;32(10):2581-3. doi: 10.1128/jcm.32.10.2581-2583.1994.
4
Comparison of blood collected in acid-citrate-dextrose and EDTA for use in human immunodeficiency virus peripheral blood mononuclear cell cultures.用于人类免疫缺陷病毒外周血单个核细胞培养的酸性枸橼酸盐葡萄糖溶液和乙二胺四乙酸抗凝血液的比较。
J Clin Microbiol. 2000 Feb;38(2):858-60. doi: 10.1128/JCM.38.2.858-860.2000.
5
Cytomegalovirus (CMV) DNA amplification from plasma compared with CMV pp65 antigen (ppUL83) detection in leukocytes for early diagnosis of symptomatic CMV infection in kidney transplant patients.肾移植患者有症状巨细胞病毒（CMV）感染的早期诊断：血浆中CMV DNA扩增与白细胞中CMV pp65抗原（ppUL83）检测的比较
Clin Diagn Virol. 1996 Nov;7(2):99-110. doi: 10.1016/s0928-0197(96)00258-9.
6
Effects of specimen collection, processing, and storage conditions on stability of human immunodeficiency virus type 1 RNA levels in plasma.样本采集、处理及储存条件对血浆中1型人类免疫缺陷病毒RNA水平稳定性的影响。
J Clin Microbiol. 1997 Nov;35(11):2886-93. doi: 10.1128/jcm.35.11.2886-2893.1997.
7
Comparison of PCR and pp65 antigenemia assay with quantitative shell vial culture for detection of cytomegalovirus in blood leukocytes from solid-organ transplant recipients.比较聚合酶链反应（PCR）、pp65抗原血症检测法与定量空斑试验培养法检测实体器官移植受者血液白细胞中巨细胞病毒的情况。
J Clin Microbiol. 1994 Apr;32(4):997-1003. doi: 10.1128/jcm.32.4.997-1003.1994.
8
Novel DNA assay for cytomegalovirus detection: comparison with conventional culture and pp65 antigenemia assay.用于巨细胞病毒检测的新型DNA检测法：与传统培养法和pp65抗原血症检测法的比较
J Clin Microbiol. 1996 Dec;34(12):3097-100. doi: 10.1128/jcm.34.12.3097-3100.1996.
9
[Comparison of the CMV antigenemia test and CMV-DNA PCR results in solid organ transplant recipients].[实体器官移植受者中巨细胞病毒抗原血症检测与巨细胞病毒DNA聚合酶链反应结果的比较]
Mikrobiyol Bul. 2016 Jan;50(1):44-52. doi: 10.5578/mb.10701.
10
Assessment of CMV load in solid organ transplant recipients by pp65 antigenemia and real-time quantitative DNA PCR assay: correlation with pp67 RNA detection.通过pp65抗原血症和实时定量DNA PCR检测评估实体器官移植受者的巨细胞病毒载量：与pp67 RNA检测的相关性
J Med Virol. 2004 Sep;74(1):78-84. doi: 10.1002/jmv.20149.

引用本文的文献

1
Biographical Feature: Marie-Louise Landry, M.D.人物特写：玛丽 - 路易丝·兰德里医学博士
J Clin Microbiol. 2019 Oct 23;57(11). doi: 10.1128/JCM.01013-19. Print 2019 Nov.
2
Development of budesonide microparticles using spray-drying technology for pulmonary administration: design, characterization, in vitro evaluation, and in vivo efficacy study.喷雾干燥技术制备布地奈德微粒用于肺部给药：设计、表征、体外评价和体内药效学研究。
AAPS PharmSciTech. 2009;10(3):993-1012. doi: 10.1208/s12249-009-9290-6. Epub 2009 Aug 1.
3
Comparison of blood collected in acid-citrate-dextrose and EDTA for use in human immunodeficiency virus peripheral blood mononuclear cell cultures.用于人类免疫缺陷病毒外周血单个核细胞培养的酸性枸橼酸盐葡萄糖溶液和乙二胺四乙酸抗凝血液的比较。
J Clin Microbiol. 2000 Feb;38(2):858-60. doi: 10.1128/JCM.38.2.858-860.2000.
4
New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients.实体器官移植受者巨细胞病毒感染及疾病的预防和治疗新策略。
Clin Microbiol Rev. 2000 Jan;13(1):83-121, table of contents. doi: 10.1128/CMR.13.1.83.
5
Quantitation of cytomegalovirus: methodologic aspects and clinical applications.巨细胞病毒的定量分析：方法学方面及临床应用
Clin Microbiol Rev. 1998 Jul;11(3):533-54. doi: 10.1128/CMR.11.3.533.
6
Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR.储存温度和时间对采用空斑小室培养法和聚合酶链反应技术检测血液标本中巨细胞病毒的定性和定量结果的影响。
J Clin Microbiol. 1997 Sep;35(9):2224-8. doi: 10.1128/jcm.35.9.2224-2228.1997.

本文引用的文献

1
Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease.巨细胞病毒定量抗原血症检测与培养方法的比较及其与临床疾病的相关性
J Clin Microbiol. 1993 Nov;31(11):2851-6. doi: 10.1128/jcm.31.11.2851-2856.1993.
2
Factors influencing detection of quantitative cytomegalovirus antigenemia.影响定量巨细胞病毒抗原血症检测的因素。
J Clin Microbiol. 1994 Mar;32(3):832-4. doi: 10.1128/jcm.32.3.832-834.1994.
3
Effect of delayed processing of blood samples on performance of cytomegalovirus antigenemia assay.血样延迟处理对巨细胞病毒抗原血症检测性能的影响。
J Clin Microbiol. 1994 Apr;32(4):1119-20. doi: 10.1128/jcm.32.4.1119-1120.1994.
4
Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.通过空斑试验、pp65抗原血症试验和聚合酶链反应比较肝素和乙二胺四乙酸（EDTA）转运管在白细胞中检测巨细胞病毒的效果
J Clin Microbiol. 1994 Oct;32(10):2581-3. doi: 10.1128/jcm.32.10.2581-2583.1994.
5
Effect of delayed specimen processing on cytomegalovirus antigenemia test results.标本处理延迟对巨细胞病毒抗原血症检测结果的影响。
J Clin Microbiol. 1995 Jan;33(1):257-9. doi: 10.1128/jcm.33.1.257-259.1995.
6
Effects of anticoagulants and storage on granulocyte function in bank blood.
Blood. 1974 Feb;43(2):207-17.
7
Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia.通过检测病毒血症、抗原血症和DNA血症监测心脏移植受者的人巨细胞病毒感染及更昔洛韦治疗情况。
J Infect Dis. 1991 Sep;164(3):488-98. doi: 10.1093/infdis/164.3.488.
8
Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction.
Transplantation. 1992 Aug;54(2):193-8. doi: 10.1097/00007890-199208000-00001.