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通过空斑试验、pp65抗原血症试验和聚合酶链反应比较肝素和乙二胺四乙酸（EDTA）转运管在白细胞中检测巨细胞病毒的效果

Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.

作者信息

Storch G A, Gaudreault-Keener M, Welby P C

机构信息

Edward Mallinckrodt Department of Pediatrics, Washington University School of Medicine, St. Louis, Missouri 63110.

出版信息

J Clin Microbiol. 1994 Oct;32(10):2581-3. doi: 10.1128/jcm.32.10.2581-2583.1994.

DOI:10.1128/jcm.32.10.2581-2583.1994
PMID:7814504
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC264108/
Abstract

The anticoagulants heparin and EDTA were compared for inhibitory effects on the detection of cytomegalovirus from washed leukocytes in specimen transport tubes. Evaluation was made by the centrifugation/shell vial culture technique, the pp65 antigenemia assay, and PCR. For each assay, the results with heparin and EDTA were equivalent.

摘要

对比了抗凝剂肝素和乙二胺四乙酸（EDTA）对在标本运输管中从洗涤后的白细胞检测巨细胞病毒的抑制作用。通过离心/空斑小瓶培养技术、pp65抗原血症检测法和聚合酶链反应（PCR）进行评估。对于每种检测方法，使用肝素和EDTA的结果相当。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38a/264108/9bc8635695d2/jcm00010-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38a/264108/9bc8635695d2/jcm00010-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e38a/264108/9bc8635695d2/jcm00010-0252-a.jpg

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Comparison of heparin and EDTA transport tubes for detection of cytomegalovirus in leukocytes by shell vial assay, pp65 antigenemia assay, and PCR.通过空斑试验、pp65抗原血症试验和聚合酶链反应比较肝素和乙二胺四乙酸（EDTA）转运管在白细胞中检测巨细胞病毒的效果
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2
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J Virol Methods. 1993 Apr;42(1):127-30. doi: 10.1016/0166-0934(93)90184-s.
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