Ghim S Y, Choi S K, Shin B S, Park S H
Bacterial Molecular Genetics RU, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Taejon, Korea.
DNA Res. 1998 Apr 30;5(2):121-6. doi: 10.1093/dnares/5.2.121.
As part of the Bacillus subtilis genome sequencing project, we determined the complete nucleotide sequence of an 8000-bp fragment downstream of the sspC gene (184 degrees) of the B. subtilis 168 chromosome. The sequence analysis shows that the sspC gene is located inside of the SP beta region, which differs from the current genetic map of B. subtilis 168. This region contains 12 putative ORFs (yojQ through yojZ and sspC). A homology search for the deduced products of the ORFs shows significant similarities to enzymes involved in deoxyribonucleotide metabolism: ribonucleotide reductase (Nrd) E, NrdF, thioredoxin and dUTPase. Interestingly, this DNA fragment includes two split genes, yojP containing conserved motifs of an intein and yojQ and yojS with an 808-bp intervening sequence for a putative intron structure. In addition, the yojR gene includes a putative new DNA replication terminator.
作为枯草芽孢杆菌基因组测序项目的一部分，我们测定了枯草芽孢杆菌168染色体sspC基因（184°）下游一个8000 bp片段的完整核苷酸序列。序列分析表明，sspC基因位于SPβ区域内，这与枯草芽孢杆菌168的当前遗传图谱不同。该区域包含12个推定的开放阅读框（从yojQ到yojZ以及sspC）。对这些开放阅读框推导产物的同源性搜索显示，它们与参与脱氧核糖核苷酸代谢的酶有显著相似性：核糖核苷酸还原酶（Nrd）E、NrdF、硫氧还蛋白和dUTP酶。有趣的是，这个DNA片段包含两个分裂基因，yojP含有一个内含肽的保守基序，yojQ和yojS之间有一个808 bp的间隔序列，形成一个推定的内含子结构。此外，yojR基因包含一个推定的新的DNA复制终止子。
