枯草芽孢杆菌染色体上spo0H和rrnH之间50 kb区域的序列分析。
Sequence analysis of a 50 kb region between spo0H and rrnH on the Bacillus subtilis chromosome.
作者信息
Yasumoto K, Liu H, Jeong S M, Ohashi Y, Kakinuma S, Tanaka K, Kawamura F, Yoshikawa H, Takahashi H
机构信息
Institute of Molecular and Cellular Biosciences, University of Tokyo, Japan.
出版信息
Microbiology (Reading). 1996 Nov;142 ( Pt 11):3039-46. doi: 10.1099/13500872-142-11-3039.
The 49630 bp spo0H-rrnH region of the Bacillus subtilis genome has been fully sequenced. The sequence contains one partial and 62 complete ORFs, one partial and three complete rRNA genes and a cluster of six tRNA genes. The direction of the transcription and translation of 61 ORFs is the same as that of the movement of the replication fork. A homology search of 40 ORFs in newly determined sequence revealed that 27 of them had significant similarity to known proteins such as elongation factor G, elongation factor Tu, pseudouridine synthase I and ribsosomal proteins. Two adjacent genes, ybaD and ybaE, appeared to encode proteins belonging to the ATP-binding cassette (ABC) family.
枯草芽孢杆菌基因组中49630 bp的spo0H-rrnH区域已完成全序列测定。该序列包含1个部分开放阅读框（ORF）和62个完整的ORF、1个部分核糖体RNA（rRNA）基因和3个完整的rRNA基因以及一簇6个转运RNA（tRNA）基因。61个ORF的转录和翻译方向与复制叉移动方向相同。对新测定序列中的40个ORF进行同源性搜索发现，其中27个与已知蛋白质具有显著相似性，如延伸因子G、延伸因子Tu、假尿苷合酶I和核糖体蛋白。两个相邻基因ybaD和ybaE似乎编码属于ATP结合盒（ABC）家族的蛋白质。
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