Bertoletti A, D'Elios M M, Boni C, De Carli M, Zignego A L, Durazzo M, Missale G, Penna A, Fiaccadori F, Del Prete G, Ferrari C
Cattedra Malattie infective, Università di Pama, Italy.
Gastroenterology. 1997 Jan;112(1):193-9. doi: 10.1016/s0016-5085(97)70235-x.
BACKGROUND & AIMS: The cytokine pattern secreted by T cells at the site of viral replication may influence the final outcome of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. The aim of this study was to assess whether a cytokine imbalance oriented toward T helper (Th) 1 or Th2-type responses may play a role in chronic hepatitis B or C.
Production of interferon (IFN)-gamma, interleukin (IL)-4, and IL-5 by wide series of T-cell clones derived from the liver of 6 patients with chronic hepatitis B (291 clones) and 9 patients with chronic hepatitis C (260 clones) was studied. T-cell clones were generated by limiting dilution from freshly isolated mononuclear cells derived from liver tissue to give a reliable representation of the intrahepatic inflammatory infiltrates.
The majority of liver-infiltrating T cells in chronic hepatitis C were Th1 cells able to secrete IFN-gamma but unable to secrete IL-4 or IL-5, whereas in hepatitis B, most CD4+ and CD8+ liver T cells were ThO-like cells able to produce not only IFN-gamma but also IL-4 and IL-5.
The different cytokine profiles of T cells within the liver in chronic HBV and HCV infections illustrate a different behavior of the local immune response in these two infections that may have pathogenetic implications.
T细胞在病毒复制部位分泌的细胞因子模式可能会影响乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）感染的最终结果。本研究旨在评估偏向于辅助性T细胞（Th）1型或Th2型应答的细胞因子失衡是否在慢性乙型或丙型肝炎中起作用。
研究了从6例慢性乙型肝炎患者（291个克隆）和9例慢性丙型肝炎患者（260个克隆）的肝脏中获得的大量T细胞克隆产生干扰素（IFN）-γ、白细胞介素（IL）-4和IL-5的情况。通过有限稀释从新鲜分离的肝脏组织单核细胞中产生T细胞克隆，以可靠地代表肝内炎性浸润情况。
慢性丙型肝炎中大多数肝浸润性T细胞是能够分泌IFN-γ但不能分泌IL-4或IL-5的Th1细胞，而在乙型肝炎中，大多数CD4+和CD8+肝T细胞是类似Th0的细胞，不仅能产生IFN-γ，还能产生IL-4和IL-5。
慢性HBV和HCV感染时肝脏内T细胞的不同细胞因子谱说明了这两种感染中局部免疫反应的不同行为，这可能具有致病意义。
