Mason G F, Behar K L, Lai J C
Department of Medicine, University of Alabama at Birmingham 35294-4470, USA.
Metab Brain Dis. 1996 Dec;11(4):283-313. doi: 10.1007/BF02029492.
As studies of brain metabolism grow in complexity, investigators turn increasingly to nuclear magnetic resonance spectroscopy combined with 13C isotopic labeling. The unique ability to detect labeling non-destructively in specific carbon positions of individual compounds has opened the way to investigate brain metabolism in systems ranging from cellular preparations to the human brain in vivo. This review is written for investigators whose backgrounds do not include detailed knowledge of principles of nuclear magnetic resonance. Its purpose is to show the wide array of NMR techniques for 13C detection that are available for application in different systems to study aspects of brain metabolism, such as metabolic compartmentation and measurements of the tricarboxylic acid cycle rate in vivo. Basic NMR concepts are explained, and, because each detection method possesses specific advantages to address the requirements of different experimental goals, basic explanations and examples are given for each technique. The review should provide readers with a basic understanding of the methods of 13C detection by NMR and assess which of the methods are most applicable to the particular issues they may face in their own research.
随着脑代谢研究的复杂性不断增加，研究人员越来越多地转向结合了¹³C同位素标记的核磁共振波谱法。在单个化合物的特定碳位置无损检测标记的独特能力，为研究从细胞制剂到活体人脑等各种系统中的脑代谢开辟了道路。这篇综述是为那些背景知识中不包括核磁共振原理详细知识的研究人员撰写的。其目的是展示可用于不同系统以研究脑代谢方面（如代谢区室化和体内三羧酸循环速率测量）的用于¹³C检测的各种核磁共振技术。文中解释了基本的核磁共振概念，并且由于每种检测方法都具有满足不同实验目标要求的特定优势，因此针对每种技术都给出了基本解释和示例。这篇综述应使读者对通过核磁共振进行¹³C检测的方法有基本的了解，并评估哪些方法最适用于他们自己研究中可能面临的特定问题。
