一种基于核酸序列扩增系统用于检测单核细胞增生李斯特菌hlyA序列。
A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences.
作者信息
Blais B W, Turner G, Sooknanan R, Malek L T
机构信息
Laboratory Services Division, Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada.
出版信息
Appl Environ Microbiol. 1997 Jan;63(1):310-3. doi: 10.1128/aem.63.1.310-313.1997.
Abstract
A nucleic acid sequence-based amplification system primarily targeting mRNA from the Listeria monocytogenes hlyA gene was developed. This system enabled the detection of low numbers (< 10 CFU/g) of L. monocytogenes cells inoculated into a variety of dairy and egg products after 48 h of enrichment in modified listeria enrichment broth.
摘要
开发了一种基于核酸序列的扩增系统，主要针对单核细胞增生李斯特菌hlyA基因的mRNA。该系统能够在改良李斯特菌增菌肉汤中富集48小时后，检测接种到各种乳制品和蛋类产品中的低数量（<10 CFU/g）单核细胞增生李斯特菌细胞。
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