Ohfuji S, Osaki M, Tsujitani S, Ikeguchi M, Sairenji T, Ito H
First Department of Pathology, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Japan.
Int J Cancer. 1996 Dec 11;68(6):710-5. doi: 10.1002/1097-0215(19961211)68:6<710::aid-ijc2910680602>3.0.co;2-6.
In order to understand the character of Epstein-Barr virus (EBV)-associated gastric carcinoma with lymphoid stroma (GCLS), we examined cell proliferation and death in comparison with gastric carcinoma without prominent lymphoid stroma (conventional gastric carcinoma). The EBV-encoded small RNA I (EBER-1) and apoptotic cells were examined by in situ hybridization (ISH) and by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labeling (TUNEL), respectively, on formalin-fixed paraffin-embedded surgical specimens from 22 GCLSs and 23 conventional gastric carcinomas. Immunostaining was performed for the detection of Ki-67 antigen (Ki-67), P53 protein (P53) and bcl-2 oncoprotein (BCL-2). The percentages of apoptotic and Ki-67-positive cells were expressed as apoptotic indices (AI) and Ki-67 labeling indices (KI), respectively. EBER-1 was detected in 19 (86%) GCLSs in contrast to none in conventional carcinomas. Mean AI was 1.8 +/- 0.6 in the EBER-1-positive GCLSs and 3.3 +/- 1.7 in the conventional gastric carcinomas, the value being significantly lower in the former (p < 0.01). Mean KI was 40.0 +/- 10.8 in the GCLSs and 48.5 +/- 9.1 in the conventional gastric carcinomas, the value being significantly lower in the former (p < 0.05). P53 expression showed no significant difference between the 2 categories of carcinoma.
为了解伴有淋巴样间质的爱泼斯坦-巴尔病毒（EBV）相关胃癌（GCLS）的特征，我们将其与无明显淋巴样间质的胃癌（传统胃癌）相比较，检测了细胞增殖和死亡情况。分别采用原位杂交（ISH）检测EBV编码的小RNA I（EBER-1），采用末端脱氧核苷酸转移酶（TdT）介导的dUTP-生物素缺口末端标记法（TUNEL）检测凋亡细胞，检测对象为22例GCLS和23例传统胃癌的福尔马林固定石蜡包埋手术标本。进行免疫染色以检测Ki-67抗原（Ki-67）、P53蛋白（P53）和bcl-2癌蛋白（BCL-2）。凋亡细胞和Ki-67阳性细胞的百分比分别表示为凋亡指数（AI）和Ki-67标记指数（KI）。19例（86%）GCLS检测到EBER-1，而传统癌中无一例检测到。EBER-1阳性的GCLS平均AI为1.8±0.6，传统胃癌为3.3±1.7，前者显著更低（p<0.01）。GCLS的平均KI为40.0±10.8，传统胃癌为48.5±9.1，前者显著更低（p<0.05）。两类癌之间P53表达无显著差异。
