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水稻中一个2型类金属硫蛋白基因的RNA表达模式

RNA expression patterns of a type 2 metallothionein-like gene from rice.

作者信息

Hsieh H M, Liu W K, Chang A, Huang P C

机构信息

Department of Life Science, National Tsing Hua University, Hsin-chu, Taiwan, ROC.

出版信息

Plant Mol Biol. 1996 Nov;32(3):525-9. doi: 10.1007/BF00019104.

Abstract

A type 2 metallothionein-like gene from rice, OsMT-2 (Oryza sativa metallothionein-like gene-2), was isolated in its cDNA form and sequenced. By northern analyses OsMT-2 expression was shown to be induced under stress by sucrose starvation, heat shock and, to a lesser extent, abscisic acid, but not excess metals, including copper. Its response to sucrose starvation was transient and different from OsMT-1, a type 1 metallothionein-like gene of rice inducible by copper. These results suggest that while OsMT-2 is also involved in cellular response to stress, its function may be complementary to that of OsMT-1.

摘要

从水稻中分离出一种2型类金属硫蛋白基因,即OsMT - 2(水稻类金属硫蛋白基因-2),并对其cDNA形式进行了测序。通过Northern分析表明,OsMT - 2的表达在蔗糖饥饿、热激胁迫下被诱导,在较小程度上也受脱落酸诱导,但不受包括铜在内的过量金属诱导。它对蔗糖饥饿的反应是短暂的,且与OsMT - 1不同,OsMT - 1是水稻中一种受铜诱导的1型类金属硫蛋白基因。这些结果表明,虽然OsMT - 2也参与细胞对胁迫的反应,但其功能可能与OsMT - 1互补。

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