Lara P C, Russell N S, Smolders I J, Bartelink H, Begg A C, Coco-Martin J M
Division of Experimental Therapy, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Int J Radiat Biol. 1996 Dec;70(6):683-92. doi: 10.1080/095530096144572.
The aim of this study was to determine whether significant inter-individual differences exist between skin fibroblast strains obtained from radiotherapy patients in both radiation-induced differentiation and collagen production in vitro, for use as potential parameters for a predictive assay for fibrosis following radiotherapy in patients. Morphological cell differentiation was determined 7 days after irradiation in seven early-passage primary human fibroblast cell strains and correlated with cell survival. Collagen production was measured in two cell strains by flow cytometry and incorporation of 3H-proline. There was a wide variation in the extent of radiation-induced differentiation for the seven cell strains, each showing a dose-related increase. The correlation between induced differentiation and cell survival was poor (r = 0.64) but statistically significant (p < 0.01). Collagen synthesis increased 7 days after irradiation for one cell strain (HF-48), as measured by incorporation of 3H-proline, but not in radiation sensitive AT-1 cells. The collagen I content of the two cell strains was assessed by flow cytometry but no significant differences were observed between the strains tested or with increasing dose. In conclusion, marked variations in radiation-induced fibroblast differentiation were observed between patients, this being an important criterion for a predictive assay.
本研究的目的是确定从放疗患者获得的皮肤成纤维细胞株在体外辐射诱导分化和胶原蛋白产生方面是否存在显著的个体间差异，以作为患者放疗后纤维化预测检测的潜在参数。在七种早期传代的原代人成纤维细胞株中，照射后7天测定细胞形态分化，并与细胞存活情况相关联。通过流式细胞术和3H-脯氨酸掺入法在两种细胞株中测量胶原蛋白的产生。七种细胞株在辐射诱导分化程度上存在很大差异，每种细胞株均显示出与剂量相关的增加。诱导分化与细胞存活之间的相关性较差（r = 0.64），但具有统计学意义（p < 0.01）。通过3H-脯氨酸掺入法测定，照射后7天一种细胞株（HF-48）的胶原蛋白合成增加，但辐射敏感的AT-1细胞中未增加。通过流式细胞术评估两种细胞株的I型胶原蛋白含量，但在所测试的细胞株之间或随着剂量增加均未观察到显著差异。总之，在患者之间观察到辐射诱导的成纤维细胞分化存在明显差异，这是预测检测的一个重要标准。
