Wood G W, Hausmann E, Choudhuri R
Department of Pathology, University of Kansas Medical Center, Kansas City 66160-7410, USA.
Mol Reprod Dev. 1997 Jan;46(1):62-9; discussion 69-70. doi: 10.1002/(SICI)1098-2795(199701)46:1<62::AID-MRD10>3.0.CO;2-5.
It has been previously demonstrated that macrophage colony stimulating factor (CSF-1) is produced by uterine epithelial cells in response to estrogen and progesterone. Studies in normal and op/op mice demonstrated that accumulation of a portion of the uterine macrophage population could be attributed to the chemotactic properties of CSF-1. Op/op mice exhibit greatly reduced rates of fertility, but successful pregnancy is not completely blocked. Also, uteri from op/op mice are not completely macrophage deficient. There are two possible explanations for this. One is that not all tissue macrophages are recruited from the bone marrow pool; some may be derived from primitive mesenchyme. Alternatively, tissue macrophages may be recruited from the bone marrow pool through expression of other type 1 chemokines such as JE, RANTES, MIP-1 alpha, MIP-1 beta, IP-10, and KC. Both RANTES and JE are expressed at higher levels than CSF-1 during early pregnancy. The variable expression and relative role of these various chemokines in pregnancy was addressed by measuring mRNA expression during the first 8 days of pregnancy and in a pseudopregnant model. The expression of these various genes relative to macrophage numbers and macrophage distribution will be discussed. The relative role of these various factors in preparing the uterus for blastocyst implantation will be discussed.
先前已经证明，巨噬细胞集落刺激因子（CSF-1）由子宫上皮细胞在雌激素和孕酮的作用下产生。对正常和op/op小鼠的研究表明，子宫巨噬细胞群体的一部分积累可归因于CSF-1的趋化特性。op/op小鼠的生育能力大大降低，但成功怀孕并未完全受阻。此外，op/op小鼠的子宫并非完全缺乏巨噬细胞。对此有两种可能的解释。一种是并非所有组织巨噬细胞都从骨髓池中募集；有些可能源自原始间充质。另一种可能是，组织巨噬细胞可能通过其他1型趋化因子如JE、RANTES、MIP-1α、MIP-1β、IP-10和KC的表达从骨髓池中募集。在妊娠早期，RANTES和JE的表达水平均高于CSF-1。通过测量妊娠前8天和假孕模型中的mRNA表达，探讨了这些不同趋化因子在妊娠中的可变表达及其相对作用。将讨论这些不同基因的表达与巨噬细胞数量和巨噬细胞分布的关系。还将讨论这些不同因素在为囊胚着床准备子宫过程中的相对作用。
