Louis N, Fender P, Barge A, Kitts P, Chroboczek J
Institut de Biologie Structurale, Grenoble, France.
J Virol. 1994 Jun;68(6):4104-6. doi: 10.1128/JVI.68.6.4104-4106.1994.
The adenovirus fiber appears as a long, thin projection terminated by a knob (head). The fiber consists of a trimeric protein whose head domain is thought to interact with cell receptors. The head part (amino acids 388 to 582) of adenovirus type 2 fiber was produced in a baculovirus expression system. The purified protein was shown to cross-link into trimers. It was very resistant to proteolytic attack and seemed to attain a high degree of compactness. The head domain efficiently inhibited attachment of adenovirus to receptors on the surface of HeLa cells, thereby confirming the hypothesis that the head domain interacts with viral receptors.
腺病毒纤维呈现为一种长而细的突起，末端为一个球状结构（头部）。该纤维由一种三聚体蛋白组成，其头部结构域被认为与细胞受体相互作用。2型腺病毒纤维的头部部分（氨基酸388至582）在杆状病毒表达系统中产生。纯化后的蛋白显示可交联形成三聚体。它对蛋白水解攻击具有很强的抗性，似乎达到了高度的紧密性。头部结构域有效地抑制了腺病毒与HeLa细胞表面受体的附着，从而证实了头部结构域与病毒受体相互作用的假说。
