Sánchez M J, Holmes A, Miles C, Dzierzak E
National Institute for Medical Research, Mill Hill, London, United Kingdom.
Immunity. 1996 Dec;5(6):513-25. doi: 10.1016/s1074-7613(00)80267-8.
At day 10 in mouse gestation, the intraembryonic aorta-gonads-mesonephros (AGM) region generates the first definitive hematopoietic stem cells (HSCs) of the adult blood system. By 11 days postcoitum, the liver contains such HSCs. While HSCs of the adult bone marrow and late-stage fetal liver have been extensively characterized for cell surface markers, there has been no phenotypic description of the first HSCs during embryo development. We report here the temporal cell surface phenotype of HSCs from the AGM region and early fetal liver and show that all HSCs reside in the c-kit+ population. c-kit+ HSCs from AGM and liver are mainly CD34+ and in the AGM are in both Mac-1+ and Mac-1 fractions. These results demonstrate that during mouse ontogeny the first definitive HSCs are similar in cell surface phenotype to the HSCs of adult bone marrow but that spatial localization and developmental time are critical factors in the phenotypic assessment of this functional cell population.
在小鼠妊娠第10天，胚胎内的主动脉-性腺-中肾（AGM）区域产生了成年血液系统中的第一批定型造血干细胞（HSC）。到交配后11天，肝脏中含有此类造血干细胞。虽然成年骨髓和晚期胎儿肝脏中的造血干细胞已针对细胞表面标志物进行了广泛表征，但胚胎发育过程中第一批造血干细胞尚无表型描述。我们在此报告AGM区域和早期胎儿肝脏中造血干细胞的时间性细胞表面表型，并表明所有造血干细胞都存在于c-kit+群体中。来自AGM和肝脏的c-kit+造血干细胞主要为CD34+，在AGM中同时存在于Mac-1+和Mac-1部分。这些结果表明，在小鼠个体发育过程中，第一批定型造血干细胞在细胞表面表型上与成年骨髓中的造血干细胞相似，但空间定位和发育时间是该功能细胞群体表型评估的关键因素。
