Shiota G, Yamada S, Kawasaki H
Second Department of Internal Medicine, Tottori University, Faculty of Medicine, Yonago, Japan.
Res Commun Mol Pathol Pharmacol. 1996 Nov;94(2):141-6.
Gomisin A (Go), a lignan component of shizandra fruits, protects the liver from injury by acetaminophen (AAP). One of its possible mechanisms is supposed to be related to the suppression of lipid peroxidation. Since hepatocyte growth factor (HGF) was reported to prevent hepatotoxin-induced liver damage, we tested HGF as the intermediary of Go effects. Simultaneous analyses of HGF mRNA expression and liver histology in rats were performed at 6 and 24 hr after treatment with AAP, Go or both. HGF mRNA rapidly expressed at 6 hr after Go treatment, while no HGF mRNA was observed at 6 hr after AAP treatment. Induction of HGF mRNA at 24 hr was observed after treatment with AAP or AAP plus Go. Histological findings indicate that massive necrosis and vacuolization in the liver of rats treated with both AAP and Go were reduced in comparison with rats treated with AAP only. These data suggest that Go rapidly induces HGF mRNA through different mechanisms from AAP-induced liver injury.
五味子果实中的木脂素成分五味子甲素（Go）可保护肝脏免受对乙酰氨基酚（AAP）的损伤。其一种可能的机制被认为与抑制脂质过氧化有关。由于据报道肝细胞生长因子（HGF）可预防肝毒素诱导的肝损伤，我们将HGF作为Go作用的中介进行了检测。在用AAP、Go或两者处理后6小时和24小时，对大鼠的HGF mRNA表达和肝脏组织学进行了同步分析。Go处理后6小时HGF mRNA迅速表达，而AAP处理后6小时未观察到HGF mRNA。用AAP或AAP加Go处理后24小时观察到HGF mRNA的诱导。组织学结果表明，与仅用AAP处理的大鼠相比，用AAP和Go处理的大鼠肝脏中的大量坏死和空泡化减少。这些数据表明，Go通过与AAP诱导的肝损伤不同的机制快速诱导HGF mRNA。
