孕妇宫颈黏液中的细胞因子浓度。

Cytokines concentrations in the cervical mucus of pregnant women.

作者信息

Sagawa T, Furuta I, Negishi H, Kishida T, Begum S, Fujimoto S

机构信息

Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, Japan.

出版信息

J Obstet Gynaecol Res. 1996 Oct;22(5):517-22. doi: 10.1111/j.1447-0756.1996.tb01065.x.

DOI:10.1111/j.1447-0756.1996.tb01065.x

PMID:8987336

Abstract

OBJECTIVE

Interleukin-1 alpha, interleukin-1 beta, interleukin-6 and interleukin-8 in specimens of cervical mucus were assayed to assess defense mechanisms against infection in the cervical canal during pregnancy.

METHODS

Two hundred and thirty-nine pregnant women who attended the obstetrics outpatient clinic in our hospital were included as subjects. Cervical mucus was collected with a newly developed collection swab, and cytokines and granulocyte elastase in the samples of cervical mucus were quantified using enzyme immunoassay kits. The 1-factor ANOVA, the Kruskal-Wallis test, and scheffé's F were used for statistical analysis.

RESULTS

Interleukin-1 alpha, interleukin-1 beta and interleukin-8 levels rose significantly as the concentrations of granulocyte elastase increased. High concentrations of interleukin-1 alpha (75.36 +/- 19.93 pg/ml), interleukin-1 beta (1005.29 +/- 238.75 pg/ml) and interleukin-8 (4822.4 +/- 633.2 pg/ml) were found in the specimens from the group of high granulocyte elastase levels.

CONCLUSIONS

These cytokines might be involved in defense mechanisms against ascending infection from the vagina.

摘要

目的

检测宫颈黏液标本中的白细胞介素-1α、白细胞介素-1β、白细胞介素-6和白细胞介素-8，以评估孕期宫颈管抗感染的防御机制。

方法

选取我院产科门诊就诊的239例孕妇作为研究对象。采用新研制的采集拭子收集宫颈黏液，并用酶免疫分析试剂盒对宫颈黏液样本中的细胞因子和粒细胞弹性蛋白酶进行定量检测。采用单因素方差分析、Kruskal-Wallis检验和Scheffé's F检验进行统计学分析。

结果

随着粒细胞弹性蛋白酶浓度升高，白细胞介素-1α、白细胞介素-1β和白细胞介素-8水平显著升高。在粒细胞弹性蛋白酶水平高的组标本中发现高浓度白细胞介素-1α（75.36±19.93 pg/ml）、白细胞介素-1β（1005.29±238.75 pg/ml）和白细胞介素-8（4822.4±633.2 pg/ml）。