Fukushima Y, Kawata Y, Onda T, Kitagawa M
Nestle Japan Ltd., Scientific Liaison Office, Tokyo,
Am J Clin Nutr. 1997 Jan;65(1):30-5. doi: 10.1093/ajcn/65.1.30.
beta-Lactoglobulin and ovalbumin in mature human milk in healthy lactating Japanese women (n = 24) were determined by using an enzyme-linked immunosorbent assay. Subjects consumed > or = +200 mL cow milk/d for 1 wk before the sampling day and exactly 200 mL cow milk on the morning of the sampling day. beta-Lactoglobulin was detected (> 0.1 microgram/L) in breast milk in 15 of the 24 subjects (62.5%), with a maximum concentration of 16.5 micrograms/L. Ovalbumin was detected in only two subjects (8.3%) after the subjects followed their usual diet. beta-Lactoglobulin concentrations were low in the subjects whose cow milk consumption during the entire lactating period was low, even though all subjects consumed the same amount of cow milk before sampling. This result suggests that beta-lactoglobulin concentrations in breast milk are related to long-term consumption of cow milk. Amounts of food antigens in breast milk may be controlled by modifying the daily maternal diet.
采用酶联免疫吸附测定法对24名健康哺乳期日本女性成熟母乳中的β-乳球蛋白和卵清蛋白进行了测定。在采样日前1周，受试者每天饮用≥200 mL牛奶，采样日当天早晨饮用200 mL牛奶。24名受试者中有15名（62.5%）母乳中检测到β-乳球蛋白（>0.1微克/升），最高浓度为16.5微克/升。在受试者遵循日常饮食后，仅两名受试者（8.3%）检测到卵清蛋白。尽管所有受试者在采样前饮用相同量的牛奶，但在整个哺乳期牛奶摄入量较低的受试者中，β-乳球蛋白浓度较低。这一结果表明，母乳中β-乳球蛋白的浓度与长期饮用牛奶有关。母乳中食物抗原的量可通过调整母亲日常饮食来控制。
