RNA聚合酶II转录起始：结构视角
RNA polymerase II transcription initiation: a structural view.
作者信息
Nikolov D B, Burley S K
机构信息
Laboratories of Molecular Biophysics, The Rockefeller University, New York, NY 10021, USA.
出版信息
Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):15-22. doi: 10.1073/pnas.94.1.15.
Abstract
In eukaryotes, RNA polymerase II transcribes messenger RNAs and several small nuclear RNAs. Like RNA polymerases I and III, polymerase II cannot act alone. Instead, general initiation factors [transcription factor (TF) IIB, TFIID, TFIIE, TFIIF, and TFIIH] assemble on promoter DNA with polymerase II, creating a large multiprotein-DNA complex that supports accurate initiation. Another group of accessory factors, transcriptional activators and coactivators, regulate the rate of RNA synthesis from each gene in response to various developmental and environmental signals. Our current knowledge of this complex macromolecular machinery is reviewed in detail, with particular emphasis on insights gained from structural studies of transcription factors.
摘要
在真核生物中，RNA聚合酶II转录信使RNA和几种小核RNA。与RNA聚合酶I和III一样，聚合酶II不能单独起作用。相反，通用起始因子[转录因子（TF）IIB、TFIID、TFIIE、TFIIF和TFIIH]与聚合酶II一起在启动子DNA上组装，形成一个大型多蛋白-DNA复合物，支持精确起始。另一组辅助因子，即转录激活因子和共激活因子，响应各种发育和环境信号，调节每个基因的RNA合成速率。本文详细综述了我们目前对这种复杂大分子机制的认识，特别强调了从转录因子结构研究中获得的见解。
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