系统性红斑狼疮中的抗双链DNA抗体：IgM类抗体的检测及其临床意义

Anti-double stranded DNA antibodies in systemic lupus erythematosus: detection and clinical relevance of IgM-class antibodies.

作者信息

Bootsma H, Spronk P E, Hummel E J, de Boer G, ter Borg E J, Limburg P C, Kallenberg C G

机构信息

Department of Internal Medicine, University Hospital Groningen, The Netherlands.

出版信息

Scand J Rheumatol. 1996;25(6):352-9. doi: 10.3109/03009749609065646.

DOI:10.3109/03009749609065646

PMID:8996469

Abstract

We determined the discriminative value of the Farr assay in comparison to ELISA and Crithidia luciliae immunofluorescence assay (IFT) for detecting anti-dsDNA antibodies as a diagnostic tool for systemic lupus erythematosus (SLE). Special attention was paid to the diagnostic significance of IgM-class anti-dsDNA. Sera were analyzed from 74 patients with SLE, 257 patients with other auto-immune diseases, and 50 healthy controls. All sera were tested for anti-dsDNA using the IFT (anti-total immunoglobulin conjugate), ELISA (anti-IgG and anti-IgM conjugates), and the 125I Farr assay. Specificity and sensitivity for a diagnosis of SLE appeared to be highest for the Farr. All SLE sera with IgM-class anti-dsDNA without IgG-class anti-dsDNA as detected by ELISA, were positive when tested by the Farr assay. In contrast, most of the sera with IgM-class anti-dsDNA as detected by ELISA from patients with diseases other than SLE were negative when tested by Farr assay.

摘要

我们确定了Farr试验相对于酶联免疫吸附测定（ELISA）和利什曼原虫免疫荧光试验（IFT）在检测抗双链DNA（dsDNA）抗体作为系统性红斑狼疮（SLE）诊断工具方面的鉴别价值。特别关注了IgM类抗dsDNA的诊断意义。分析了74例SLE患者、257例其他自身免疫性疾病患者和50例健康对照者的血清。所有血清均使用IFT（抗总免疫球蛋白结合物）、ELISA（抗IgG和抗IgM结合物）和125I Farr试验检测抗dsDNA。Farr试验对SLE诊断的特异性和敏感性似乎最高。ELISA检测到的所有无IgG类抗dsDNA的IgM类抗dsDNA的SLE血清，经Farr试验检测均为阳性。相比之下，ELISA检测到的大多数非SLE疾病患者的IgM类抗dsDNA血清，经Farr试验检测为阴性。