Chandler J, Wilson A, Dean C
John Innes Centre, Norwich Research Park, Norwich, UK.
Plant J. 1996 Oct;10(4):637-44. doi: 10.1046/j.1365-313x.1996.10040637.x.
Flowering in many plant species is accelerated by a long period of cold temperature, known as a vernalization period. This research investigates how this cold temperature signal is perceived by plant cells and the mechanism by which it influences the transition to flowering. Mutagenesis of the late-flowering, vernalization-responsive, Arabidopsis mutant, fca, has yielded five independent mutations (termed vrn mutations) conferring an altered vernalization response. Allelism tests showed that these mutations fall into at least three complementation groups defining three loci named VRN 1, 2 and 3. The vrn1 and vrn2 mutations did not affect the acclimation response as judged by expression of cold-induced transcripts and freezing tolerance assays. vrn1-1 affected the short-day vernalization response of Landsberg erecta and reduced the vernalization response of other late-flowering Arabidopsis mutants. The acceleration of flowering by GA3 was not affected by vrn1-1. The VRN 1 locus was mapped to chromosome 3.
许多植物物种的开花会因一段长时间的低温（即春化期）而加速。本研究调查了植物细胞如何感知这种低温信号以及它影响开花转变的机制。晚花、对春化有反应的拟南芥突变体fca的诱变产生了五个独立的突变（称为vrn突变），这些突变赋予了改变的春化反应。等位性测试表明，这些突变至少分为三个互补群，定义了三个名为VRN 1、2和3的位点。根据冷诱导转录本的表达和抗冻性测定判断，vrn1和vrn2突变不影响驯化反应。vrn1-1影响了Landsberg erecta的短日春化反应，并降低了其他晚花拟南芥突变体的春化反应。GA3对开花的加速作用不受vrn1-1的影响。VRN 1位点被定位到3号染色体上。
