尿路感染致病性大肠杆菌K13抗原性多糖（K13抗原）的结构与血清学特异性
Structure and serological specificity of the K13-antigenic polysaccharide (K13 antigen) of urinary tract-infective Escherichia coli.
作者信息
Vann W F, Jann K
出版信息
Infect Immun. 1979 Jul;25(1):85-92. doi: 10.1128/iai.25.1.85-92.1979.
Abstract
The primary structure of the K13-antigenic polysaccharide (K13 antigen) of Escherichia coli O6:K13:H1 was elucidated by composition, periodate oxidation, Smith degradation, and methylation analysis. The polysaccharide consists of a repeating sequence of 3-linked ribofuranose and 7-linked 3-deoxymannooctulosonic acid (KDO). About 50% of the KDO residues are O-acetylated at position 4 or 5. Measurement of the optical rotary dispersion indicated that in aqueous solution the K13 polysaccharide assumes a secondary structure in which the carboxyl groups of KDO are engaged. The serological specificity of the K13 polysaccharide is expressed through KDO and its O-acetyl substituent, the ribose unit being antigenically silent. There are two populations of anti-K13 antibodies one directed against the charged region of the KDO and the other against the O-acetyl groups.
摘要
通过组成分析、高碘酸盐氧化、史密斯降解和甲基化分析，阐明了大肠杆菌O6:K13:H1的K13抗原多糖（K13抗原）的一级结构。该多糖由3-连接的呋喃核糖和7-连接的3-脱氧甘露辛酮糖酸（KDO）的重复序列组成。约50%的KDO残基在4或5位被O-乙酰化。旋光色散测量表明，在水溶液中K13多糖呈现出一种二级结构，其中KDO的羧基参与其中。K13多糖的血清学特异性通过KDO及其O-乙酰取代基表现出来，核糖单元在抗原性上无活性。有两类抗K13抗体，一类针对KDO的带电区域，另一类针对O-乙酰基。
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