运用聚合酶链反应（PCR）和SHARP信号检测系统对患有和未患有巨细胞病毒（CMV）疾病的人类免疫缺陷病毒感染受试者白细胞中的CMV DNA进行定量分析。
Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System.
作者信息
Boivin G, Handfield J, Murray G, Toma E, Lalonde R, Lazar J G, Bergeron M G
机构信息
Department of Microbiology, Université Laval, Quebec City, Canada.
出版信息
J Clin Microbiol. 1997 Feb;35(2):525-6. doi: 10.1128/jcm.35.2.525-526.1997.
Abstract
We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.
摘要
我们报告了一种用于定量检测多形核白细胞中巨细胞病毒（CMV）DNA载量的简单快速PCR检测方法的开发。使用该系统，在艾滋病患者中发现血液中大量CMV拷贝数与CMV疾病的存在之间存在非常好的相关性。
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