Gómez-Villamandos J C, Bautista M J, Hervás J, Carrasco L, de Lara F C, Pérez J, Wilkinson P J, Sierra M A
Departmento de Anatomía y Anatomía Patológica, Facultad de Veterinaria, Universidad de Córdoba, Spain.
J Comp Pathol. 1996 Nov;115(4):327-41. doi: 10.1016/s0021-9975(96)80069-7.
The morphological changes in platelets in acute and subacute African swine fever (ASF) and their relationship to pathogenesis were studied. Eight pigs were inoculated with a highly virulent strain of African swine fever (Malawi '83) and 14 with a moderately virulent strain (Dominican Republic '78) for ultrastructural study of platelets, monocyte/macrophages and vascular structures in the liver, spleen, lymph node, bone marrow, lung and kidney. Both viruses produced activation and degranulation of platelets from day 3 after inoculation onwards, coinciding with activation of the mononuclear phagocyte system and virus replication in monocyte/macrophages. Platelet aggregation and viscous metamorphosis of platelets were observed at 5 and 7 days after inoculation with the highly virulent strain, coinciding with endothelial alterations, but platelet aggregation was less prevalent and there was no sign of viscous metamorphosis in animals inoculated with the moderately virulent strain. Virions within platelets were observed at the final stage of acute ASF and at 5-7 days after inoculation in subacute ASF. This suggests that platelets assist in disseminating ASF virus within the body, especially in subacute infections.
研究了急性和亚急性非洲猪瘟（ASF）中血小板的形态变化及其与发病机制的关系。八头猪接种了高毒力非洲猪瘟病毒株（马拉维'83），十四头接种了中等毒力病毒株（多米尼加共和国'78），以对肝脏、脾脏、淋巴结、骨髓、肺和肾脏中的血小板、单核细胞/巨噬细胞及血管结构进行超微结构研究。两种病毒在接种后第3天起均导致血小板活化和脱颗粒，这与单核吞噬细胞系统的活化以及病毒在单核细胞/巨噬细胞中的复制同时发生。接种高毒力病毒株后第5天和第7天观察到血小板聚集和血小板粘性变形，这与内皮改变同时出现，但接种中等毒力病毒株的动物中血小板聚集较少见，且无粘性变形迹象。在急性ASF末期和亚急性ASF接种后5 - 7天观察到血小板内的病毒粒子。这表明血小板有助于在体内传播ASF病毒，尤其是在亚急性感染中。
