Carrasco L, Chàcón-M de Lara F, Martín de Las Mulas J, Gómez-Villamandos J C, Sierra M A, Villeda C J, Wilkinson P J
Departamento de Anatomía y Anatomía Patológica Comparadas, Facultad de Veterinaria, Universidad de Córdoba, Spain.
Res Vet Sci. 1997 May-Jun;62(3):199-204. doi: 10.1016/s0034-5288(97)90190-9.
In order to determine the pathogenic mechanisms involved in lymph node haemorrhages in acute African swine fever (ASF), eight pigs were inoculated with ASF virus, strain Malawi'83. Lymph node haemorrhages were observed from three days post infection (dpi) onwards, coinciding with ASF virus replication in monocytes and macrophages adjacent to stimulated endothelial cells, phagocytic stimulation of capillary and small-vessel endothelial cells, increase in the number of fenestrations of endothelial cells, and endothelial cell loss, as well as clusters of blood cells and necrotic material beneath the endothelium. Vascular lumina were blocked by platelet plugs and fibrin microthrombi. These phenomena became more marked as the disease progressed. At five dpi, virus replication was also found in circulating neutrophils. At seven dpi, lesions were more intense and were accompanied by virus replication in sinus and capillary endothelial cells, and in other cell populations including pericytes, fibroblasts, smooth muscle fibres and reticular cells. The results obtained in this study suggest that lymph node haemorrhages are related to endothelial stimulation and the onset of disseminated intravascular coagulation. Virus replication in vessel wall cells occurs only in the final stages of the disease and plays a secondary role.
为了确定急性非洲猪瘟（ASF）淋巴结出血所涉及的致病机制，用马拉维'83株非洲猪瘟病毒接种了8头猪。从感染后3天（dpi）开始观察到淋巴结出血，这与非洲猪瘟病毒在受刺激内皮细胞附近的单核细胞和巨噬细胞中的复制、毛细血管和小血管内皮细胞的吞噬刺激、内皮细胞窗孔数量增加、内皮细胞丢失以及内皮细胞下方的血细胞和坏死物质簇相吻合。血管腔被血小板凝块和纤维蛋白微血栓阻塞。随着疾病进展，这些现象变得更加明显。在5 dpi时，还在循环中性粒细胞中发现病毒复制。在7 dpi时，病变更加严重，并伴有病毒在窦状和毛细血管内皮细胞以及包括周细胞、成纤维细胞、平滑肌纤维和网状细胞在内的其他细胞群体中的复制。本研究获得的结果表明，淋巴结出血与内皮刺激和弥散性血管内凝血的发生有关。病毒在血管壁细胞中的复制仅发生在疾病的最后阶段，起次要作用。
