大肠杆菌二氢新蝶呤三磷酸差向异构酶：纯化、基因克隆及表达
Dihydroneopterin triphosphate epimerase of Escherichia coli: purification, genetic cloning, and expression.
作者信息
Haussmann C, Rohdich F, Lottspeich F, Eberhardt S, Scheuring J, Mackamul S, Bacher A
机构信息
Institut für Organische Chemie und Biochemie, Technische Universität München, Garching, Federal Republic of Germany.
出版信息
J Bacteriol. 1997 Feb;179(3):949-51. doi: 10.1128/jb.179.3.949-951.1997.
Abstract
The enzyme catalyzing the epimerization at position 2' of dihydroneopterin triphosphate was purified by a factor of about 10,000 from cell extract of Escherichia coli. The cognate gene was cloned, sequenced, expressed, and mapped to kb 2427 on the E. coli chromosome.
摘要
催化三磷酸二氢新蝶呤2'位差向异构化的酶从大肠杆菌细胞提取物中纯化了约10000倍。同源基因被克隆、测序、表达并定位到大肠杆菌染色体上的2427 kb处。
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