Topaktaş M, Rencüzoğullari E, Ila H B
Department of Biology, Faculty of Arts and Science, Cukurova University, Adana, Turkey.
Mutat Res. 1996 Dec 20;371(3-4):259-64.
In this study, the cytogenetic effects of Marshal (insecticide/nematocide) were investigated in bone marrow cells of rats. The results obtained from animals treated with Marshal were compared with the results of animals treated with ethyl carbamate (EC) and with controls. Concentrations of 12.5, 25 and 50 mg/kg b.wt. of Marshal and 100, 200 and 400 mg/kg b.wt. of EC were used and animals were sampled at three different times (6, 12 and 24 h). Marshal increased the number of chromosomal aberrations (CA) per cell, and the number of cells with abnormalities, at all concentrations and treatment times. Generally, Marshal could increase the number of the abnormal cells and the formation of CA as easily as EC. However, Marshal, at 50 mg/kg b.wt. did not increase the frequency of abnormal cells or CA as strongly as EC, at 400 mg/kg b.wt. for 6 h. Marshal also decreased the mitotic index (MI) compared with the control group. The MI was higher in the group treated with Marshal for 6 h than that treated with EC. However, the effects of Marshal and EC on the MI in the groups treated for 12 and 24 h were similar. We found that the effect of Marshal on the formation of abnormal cells and CA was dependent on concentration and treatment time.
在本研究中，对大鼠骨髓细胞中马拉硫磷（杀虫剂/杀线虫剂）的细胞遗传学效应进行了研究。将用马拉硫磷处理的动物所得到的结果与用氨基甲酸乙酯（EC）处理的动物的结果以及对照组的结果进行了比较。使用了12.5、25和50毫克/千克体重的马拉硫磷以及100、200和400毫克/千克体重的EC，并且在三个不同时间点（6、12和24小时）对动物进行采样。在所有浓度和处理时间下，马拉硫磷均增加了每个细胞的染色体畸变（CA）数量以及出现异常的细胞数量。一般来说，马拉硫磷与EC一样容易增加异常细胞的数量和CA的形成。然而，50毫克/千克体重的马拉硫磷在6小时时，增加异常细胞频率或CA的程度不如400毫克/千克体重的EC强烈。与对照组相比，马拉硫磷还降低了有丝分裂指数（MI）。用马拉硫磷处理6小时的组的MI高于用EC处理的组。然而，马拉硫磷和EC对处理12小时和24小时的组的MI的影响相似。我们发现，马拉硫磷对异常细胞形成和CA的影响取决于浓度和处理时间。
