Wittinghofer A, Nassar N
Max-Planck-Institut für Molekulare Physiologie, Dortmund, Germany.
Trends Biochem Sci. 1996 Dec;21(12):488-91. doi: 10.1016/s0968-0004(96)10064-5.
More and more effectors for the Ras-related protein superfamily are being discovered and it is emerging that these GTP-binding proteins interact with more than one effector to generate more than one cellular signal. Atomic details for the interaction of Rap/Ras with one of the effectors, the protein kinase c-Raf-1, have recently become available by X-ray structure analysis. The implications for the specificity of the signal transduction pathway, and how the GTP-dependent switch mechanism modulates the interaction with effectors will be discussed here, using Ras as a paradigm.
越来越多与Ras相关蛋白超家族的效应器被发现，并且逐渐显现出这些GTP结合蛋白与不止一种效应器相互作用以产生不止一种细胞信号。Rap/Ras与其中一种效应器——蛋白激酶c-Raf-1相互作用的原子细节最近通过X射线结构分析得以知晓。本文将以Ras作为范例，讨论信号转导途径特异性的影响，以及GTP依赖的开关机制如何调节与效应器的相互作用。
