Jodo S, Kobayashi S, Kayagaki N, Ogura N, Feng Y, Amasaki Y, Fujisaku A, Azuma M, Yagita H, Okumura K, Koike T
Department of Medicine II, Hokkaido University School of Medicine, Sapporo, Japan.
Clin Exp Immunol. 1997 Jan;107(1):89-95. doi: 10.1046/j.1365-2249.1997.d01-901.x.
There are two major forms of the Fas molecule, membranous Fas and soluble Fas (sFas). To clarify the clinical significance of sFas in autoimmune diseases, we designed a sandwich ELISA to determine serum concentrations of sFas and its molecular structure, and we then analysed the correlation between levels of sFas and laboratory findings in patients with SLE and other autoimmune diseases. The levels of serum sFas were significantly higher in SLE patients than in subjects with other autoimmune diseases and in healthy donors, and the frequency of a positive serum sFas was much greater in SLE patients with high SLE disease activity index scores than in those with low scores. In addition, sFas-positive SLE patients showed a significant difference in various laboratory parameters from sFas-negative SLE patients. Serial measurements of serum sFas levels in SLE patients with active disease revealed that the elevated level of sFas dramatically decreased with improvement in clinical and laboratory findings, following corticosteroid therapy. We propose that the serum level of sFas can serve as an appropriate marker for evaluating SLE disease activity. Serum sFas is heterogeneous with respect to molecular structure, thus several mechanisms are involved in the generation of sFas.
Fas分子有两种主要形式，即膜 Fas 和可溶性 Fas（sFas）。为阐明 sFas 在自身免疫性疾病中的临床意义，我们设计了一种夹心酶联免疫吸附测定法来测定血清中 sFas 的浓度及其分子结构，然后分析了系统性红斑狼疮（SLE）患者及其他自身免疫性疾病患者血清 sFas 水平与实验室检查结果之间的相关性。SLE 患者血清 sFas 水平显著高于其他自身免疫性疾病患者及健康供者，且 SLE 疾病活动指数评分高的患者血清 sFas 阳性频率远高于评分低的患者。此外，sFas 阳性的 SLE 患者与 sFas 阴性的 SLE 患者在各项实验室参数上存在显著差异。对活动期 SLE 患者的血清 sFas 水平进行连续测量发现，在接受皮质类固醇治疗后，随着临床和实验室检查结果的改善，sFas 升高的水平显著下降。我们认为血清 sFas 水平可作为评估 SLE 疾病活动度的合适指标。血清 sFas 在分子结构上具有异质性，因此 sFas 的产生涉及多种机制。
