Wörl J, Mayer B, Neuhuber W L
Anatomisches Institut, Universität Erlangen-Nürnberg, Krankenhausstrasse 9, D-91054 Erlangen, Germany.
Cell Tissue Res. 1997 Jan;287(1):113-8. doi: 10.1007/s004410050736.
Enteric co-innervation of motor endplates in the rat esophagus was studied with confocal laser scanning and electron microscopy. Enteric fibers were demonstrated with immunocytochemistry for nitric oxide synthase, vasoactive intestinal peptide or NADPH-diaphorase histochemistry. Vagal motor terminals were identified with calcitonin gene-related peptide (CGRP) immunocytochemistry. Teloglia was stained with immuno- cytochemistry for S100, and TRITC-tagged alpha-bungarotoxin was used to delineate endplate areas in immmunofluorescence preparations. Both confocal imaging and electron microscopy revealed intimate relationships between enteric and vagal terminals on the one hand, and enteric terminals and the sarcolemma on the other. In addition, electron microscopy could point out direct apposition of a significant proportion of enteric varicosities to vagal motor terminals without intervening teloglial processes. These morphological data are compatible with pre- and postsynaptic modulatory effects of enteric neurons on vagal neuromuscular transmission in striated esophageal muscle.
采用共聚焦激光扫描和电子显微镜技术，研究大鼠食管运动终板的肠内共同支配。通过一氧化氮合酶免疫细胞化学、血管活性肠肽免疫细胞化学或NADPH - 黄递酶组织化学来显示肠纤维。用降钙素基因相关肽（CGRP）免疫细胞化学鉴定迷走运动终末。用S100免疫细胞化学对终神经胶质细胞进行染色，并用TRITC标记的α - 银环蛇毒素在免疫荧光制剂中描绘终板区域。共聚焦成像和电子显微镜均显示，一方面肠内和迷走终末之间，另一方面肠内终末和肌膜之间存在密切关系。此外，电子显微镜能够指出相当一部分肠曲张体与迷走运动终末直接并置，中间没有终神经胶质细胞突起的介入。这些形态学数据与肠神经元对横纹肌食管肌中迷走神经肌肉传递的突触前和突触后调节作用相符。
